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Overview

  • Product nameAnti-A1CF antibodySee all A1CF primary antibodies ...
  • Description
    Rabbit polyclonal to A1CF
  • Tested applicationsWB more details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 2-51 (ESNHKSGDGL SGTQKEAALR ALVQRTGYSL VQENGQRKYG GPPPGWDAAP) of Human A1CF (NP_055391).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive controlHuman fetal spleen lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab94598 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB
  • Application notesWB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 65 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • FunctionEssential component of the apolipoprotein B mRNA editing enzyme complex which is responsible for the postranscriptional editing of a CAA codon for Gln to a UAA codon for stop in APOB mRNA. Binds to APOB mRNA and is probably responsible for docking the catalytic subunit, APOBEC1, to the mRNA to allow it to deaminate its target cytosine. The complex also protects the edited APOB mRNA from nonsense-mediated decay.
    • Tissue specificityWidely expressed with highest levels in brain, liver, pancreas, colon and spleen.
    • Sequence similaritiesContains 3 RRM (RNA recognition motif) domains.
    • DomainThe RRM domains are necessary but not sufficient for binding to APOB mRNA. Additional residues in the pre-RRM and C-terminal regions are required for RNA-binding and for complementing APOBEC1 activity.
    • Cellular localizationNucleus. Endoplasmic reticulum. Cytoplasm. Predominantly nuclear where it localizes to heterochromatin. Also cytoplasmic where it is found at the outer surface of the endoplasmic reticulum (By similarity). Shuttles between the nucleus and cytoplasm. May be transported into the nucleus by the nuclear import protein TNPO2/TRN2 or by APOBEC1.

      • Target information above from: UniProt accession Q9NQ94 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
      see all
    • Alternative names
        A1CF antibodyA1CF_HUMAN antibodyACF antibody
        ACF64 antibodyACF65 antibodyApo B RNA editing protein antibodyApobec 1 complementation factor antibodyApobec 1 complementation factor (ACF) (ASP) antibodyAPOBEC 1 stimulating protein antibodyAPOBEC1 complementation factor antibodyAPOBEC1 stimulating protein antibodyAPOBEC1-stimulating protein antibodyAPOBEC1CF antibodyASP antibodyMGC163391 antibodyRP11-564C4.2 antibody
      see all

    Anti-A1CF antibody images

    • Western blot - A1CF antibody (ab94598)
      Western blot - A1CF antibody (ab94598)
      Anti-A1CF antibody (ab94598) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal spleen lysate at 10 µg

      Secondary
      HRP conjugated anti-Rabbit IgG at 1/50000 dilution

      Predicted band size : 65 kDa

    References for Anti-A1CF antibody (ab94598)

    ab94598 has not yet been referenced specifically in any publications.

    Publishing research using ab94598 ? Please let us know so that we can cite the reference in this datasheet

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