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Products:Kits/ Lysates/ Other >> Lysates >> Whole Cell Lysates >> Mouse >> Other
Overview
Product name
A20 (Mouse B lymphoma cell line) Whole Cell Lysate
General notes
Cell line: A-20 (B lymphocyte; reticulum cell sarcoma)
Growth media: RPMI 1640 medium with 2 mM L-glutamine & 10% FBS
Tested applications
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
pH: 6.80
Constituent: 5% Beta mercaptoethanol
Concentration
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Purity
Whole Cell Lysate
Lysate notes
Mouse A-20 cell lysate was prepared by homogenization in modified RIPA buffer (50 mM Tris-HCl, pH 7.4, 1% Triton X-100, 0.2% sodium deoxycholate, 0.2% sodium dodecylsulfate (SDS), 1 mM sodium ethylenediaminetetraacetate, 1 mM phenylmethylsulfonyl flouride, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Cell debris were removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% SDS, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.
Research areas
Kits/ Lysates/ Other >> Lysates >> Whole Cell Lysates >> Mouse >> Other
Background
A20 cells are derived from a reticulum cell sarcoma and are used as a cell system for isolation and extraction of histone deacetylases.
Applications
Show applications key
Our Abpromise guarantee covers the use of ab7180 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at an assay dependent dilution. (Ready to load on SDS-PAGE for Western blotting, 20 µg per lane is recommended for mini gel.)
References for A20 (Mouse B lymphoma cell line) Whole Cell Lysate (ab7180)
ab7180 has not yet been referenced specifically in any publications.
Publishing research using ab7180? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"