Anti-A2B5 antibody [105] - Stem Cell Marker (ab53521)

Overview

• Product nameAnti-A2B5 antibody [105] - Stem Cell MarkerSee all A2B5 primary antibodies ...
• Description
  Mouse monoclonal [105] to A2B5 - Stem Cell Marker
• SpecificityThis antibody reacts with a surface antigen on neurons in the retina, brain, spinal cord and dorsal root ganglia, and with all tested human neuroblastoma cell lines. It does not seem to bind with any leukemic cell lines or with bone marrow cells from patients with leukemia, nor with cells from normal bone marrow. It is cytotoxic to neurons in the presence of guinea-pig complement. Protein A binding is positive. It binds with GQ ganglioside on the plasma membrane of neurons of animals of all species tested to date (Eisenbarth, et al., 1979).
• Tested applicationsFlow Cyt, IHC-Fr, ICC/IF more details
• Species reactivity
  Reacts with: Mouse, Rat, Chicken, Human
  
• Immunogen

  Embryonic chicken retinal cells.

• Positive controlType II astrocytes, human neural progenitors.

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: PBS, pH 7.4
• Concentration information loading...
• PurityPurified IgM
• Clonality Monoclonal
• Clone number105
• MyelomaP3-x63-Ag8
• IsotypeIgM
• Light chain typekappa
• Research Areas
  • Stem Cells
  • Neural Stem Cells
  • Glial Restricted Lineage
  • Astrocyte

  • Tags & Cell Markers
  • Cell Type Markers
  • Neuroscience Markers
  • Glial

  • Neuroscience
  • Cell Type Marker
  • Neural Stem Cell marker

  • Neuroscience
  • Cell Type Marker
  • Glia marker
  • Oligodendrocyte marker

Applications

Target

• RelevanceA2B5 is a cell surface ganglioside epitope expressed in developing thymic epithelial cells, oligodendrocyte progenitors and neuroendocrine cells.
• Cellular localizationCell surface

Anti-A2B5 antibody [105] - Stem Cell Marker images

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  Immunocytochemistry/ Immunofluorescence - A2B5 antibody [105] - Stem Cell Marker (ab53521)
  ICC/IF image of ab53521 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53521, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgM (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue).
• 

  Immunocytochemistry/ Immunofluorescence - A2B5 antibody [105] - Stem Cell Marker (ab53521)This image is courtesy of an Abreview submitted by Mr Carl Hobbs
  A2B5 antibody [105] - Stem Cell Marker (ab53521) used in immunocytochemical detection on Cor1 neuronal stem cells. Cor1 neuronal stem cells were fixed in formaldehyde, permeabilized, blocked in 1% BSA for 30 mins at RT. ab53521 was incubated at 1/500 for 16 hours in TBS/BSA/azide/0.5% Triton. Secondary Antibody:anti mouse IgM conjugated: to Alexa Fluor® 488 (1/1000).

  See Abreview

• 

  Flow Cytometry - Anti-A2B5 antibody [105] - Stem Cell Marker (ab53521)
  Overlay histogram showing SH-SY5Y cells stained with ab53521 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53521, 1µg/1x10⁶ cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgM (mu chain) (ab97007) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgM [ICIGM] (ab91545, 2µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.
  
  Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.