If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »This product is covered by the Abpromise guarantee. Our scientific support team are available to answer any questions or queries - fill out an inquiry form for ab83574 for help.
Alternatively, you can search the previous enquiries about this product to see if your query has already been answered.
|
|||||||||
|
|||||||||
P {margin-top:2px;margin-bottom:2px;} Dear technical team, Our customer would like to test IF or IHC test with mouse brain dentate gyrus(DG). She searched ab94581, ab83574 and wants to confirm if they can detect dentate gyrus or not. Check this please. Best regards, |
|||||||||
ANSWER: |
Thank you for your enquiry regarding our two antibodies (ab83574 and ab94581). Generally, brain (CNS) cells express Fox1 at high levels particularly in the following region cerebral cortex, hippocampus and lateral ventricle. As the datasheets indicates, both of these antibodies have been tested for cross-reaction with mouse. However, they have not been specifically tested on mouse brain tissue sections containing dentate gyrus region (DG). Furthermore it is important to mention that ab83574 has not been characterized in our Lab for ICC/IF neither for IHC. However, we have published an Abreview on IHC-FoFr so we list this specific staining application on the datasheet. Regarding the other antibody ab94581, it has only been tested in -house in ICC/IF. No data or preliminary results are available for immunohistochemistry. I hope this helps and if I can assist further, please do not hesitate to contact me. |
||||||||
|
|||||||||
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Anti-A2BP1 / Fox1 antibody (ab83574) at 1 µg/ml (in 5% skim milk/ PBS) + MCF7 cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 43 kDa
Observed band size : 40 kDa (why is the actual band size different from the predicted?)
Additional bands at : 55 kDa. We are unsure as to the identity of these extra bands.
1
Call 01223 696 000 or contact us