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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> RNA Processing >> Splicing
Overview
Product name
Anti-A2BP1 / Fox1 antibody
See all A2BP1 / Fox1 products (3) ...
Description
Rabbit polyclonal to A2BP1 / Fox1
Tested applications
WB, ELISA, IHC-FoFrmore details
Cross reactivity
Reacts with
Mouse, Human
Predicted to work with
Cow
Immunogen
Synthetic peptide from within the N terminal amino acids 1-50: MNCEREQLRG NQEAAAAPDT MAQPYASAQF APPQNGIPAE YTAPHPHPAP, of Human A2BP1 / Fox1 (NP_001135805)
Positive control
MCF7 cell lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
-
Western blot - A2BP1 / Fox1 antibody (ab83574)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab83574 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
ELISA
Application notes
ELISA: Use at an assay dependent dilution. ELISA titre using peptide based assay: 1:62500
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 43 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
RNA-binding protein that regulates alternative splicing events by binding to 5'-UGCAUGU-3' elements. Regulates alternative splicing of tissue-specific exons and of differentially spliced exons during erythropoiesis.
Tissue specificity
Predominantly expressed in muscle and brain.
Sequence similarities
Contains 1 RRM (RNA recognition motif) domain.
Cellular localization
Nucleus. Cytoplasm.
Target information above from: UniProt accessionQ9NWB1
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- A2 BP1 antibody
- A2BP 1 antibody
- A2BP antibody
see all
Database links
- Entrez Gene: 54715 Human
- Entrez Gene: 268859 Mouse
- Omim: 605104 Human
- SwissProt: Q9NWB1 Human
- SwissProt: Q9JJ43 Mouse
- Unigene: 459842 Human
- Unigene: 370334 Mouse
Anti-A2BP1 / Fox1 antibody images:
Western blot - A2BP1 / Fox1 antibody (ab83574)
Anti-A2BP1 / Fox1 antibody (ab83574) at 1 µg/ml (in 5% skim milk/ PBS) + MCF7 cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 43 kDa
Observed band size : 40 kDa (why is the actual band size different from the predicted?)
Additional bands at : 55 kDa. We are unsure as to the identity of these extra bands.
References for Anti-A2BP1 / Fox1 antibody (ab83574)
ab83574 has not yet been referenced specifically in any publications.
Publishing research using ab83574? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"