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Western blot - A2BP1 / Fox1 antibody (ab83574)
(enlarge)
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Product Name
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A2BP1 / Fox1 antibody
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See all A2BP1 / Fox1 antibodies (2)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to A2BP1 / Fox1
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Immunogen
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Synthetic peptide from within the N terminal amino acids 1-50: MNCEREQLRG NQEAAAAPDT MAQPYASAQF APPQNGIPAE YTAPHPHPAP, of Human A2BP1 / Fox1 (NP_001135805)
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Reacts with
(species key)
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Hu, Ms
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Tested applications
(see key)
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ELISA, IHC-FoFr, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: Use at an assay dependent dilution. ELISA titre using peptide based assay: 1:62500
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 43 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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MCF7 cell lysate
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Cellular localization
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Golgi Apparatus and Nuclear
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Research areas
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Developmental Biology >> Organogenesis >> Hematopoietic system development
Neuroscience >> Neurology process >> Neurodegenerative disease >> Other
Chromatin and Nuclear Signaling >> DNA / RNA >> RNA Processing >> Splicing
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Relevance
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A2BP1 / FOX1 (ataxin 2 binding protein 1)contains a highly conserved RNP motif that is common among RNA binding proteins. It regulates alternative splicing events by binding to 5'-UGCAUGU-3' elements. A2BP1 / FOX1 regulates the alternative splicing of tissue specific expnes and of differentially spliced exons during erythropoiesis. Ataxin 2, the gene product of the SCA2 gene, is assoicated with a number of familial neurodegenerative diseases. A2BP1 / FOX1 binds to the C terminus of ataxin 2 and may constribute to the pathology of spinocerebellar ataxia type 2 (SCA2). In addition to its localisation within the nucleus, A2BP1 / FOX1 localises to the trans Golgi network with Ataxin 2.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: None
Constituents: 2% Sucrose, PBS
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Form
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Lyophilised
Add 50 µl of distilled water. Final antibody concentration is 1 mg/ml.
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this A2BP1 / Fox1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for A2BP1 / Fox1 antibody images, references, products related to ab83574 and other tools.
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A2BP1 / Fox1 antibody images:
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 Western blot - A2BP1 / Fox1 antibody (ab83574)
A2BP1 / Fox1 antibody (ab83574) at 1 µg/ml (in 5% skim milk/ PBS) + MCF7 cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 43 kDa
Observed band size : 40 kDa (why is the actual band size different from the predicted?)
Additional bands at : 55 kDa. We are unsure as to the identity of these extra bands.
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Search PubMed (MEDLINE) for references to A2BP1 / Fox1
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A2BP1 / Fox1 antibody - more information
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Customer reviews (feedback) regarding A2BP1 / Fox1 antibody |
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A2BP1 / Fox1 antibody - related products:
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A2BP1 / Fox1 antibody - other tools:
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Contact Abcam with a Technical Enquiry about A2BP1 / Fox1 antibody |
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