Anti-A2BP1 / Fox1 antibody (ab94581)

Overview

• Product nameAnti-A2BP1 / Fox1 antibodySee all A2BP1 / Fox1 primary antibodies ...
• Description
  Rabbit polyclonal to A2BP1 / Fox1
• Tested applicationsICC/IF, IHC-P, WB more details
• Species reactivity
  Reacts with: Mouse, Human
  Predicted to work with: Cow, Dog, Monkey, Orangutan
• Immunogen

  Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human A2BP1 / Fox1.

  Read Abcam's proprietary immunogen policy

• Positive controlThis antibody gave a positive signal in Mouse Forebrain Tissue and MCF7 Whole Cell lysates. This antibody gave a positive result in IHC in the following FFPE tissue: Human skeletal muscle.

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 1% BSA, PBS, pH 7.4
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Developmental Biology
  • Organogenesis
  • Hematopoietic system development

  • Epigenetics and Nuclear Signaling
  • DNA / RNA
  • RNA Processing
  • Splicing

  • Neuroscience
  • Neurology process
  • Neurodegenerative disease
  • Other

Applications

Target

• FunctionRNA-binding protein that regulates alternative splicing events by binding to 5'-UGCAUGU-3' elements. Regulates alternative splicing of tissue-specific exons and of differentially spliced exons during erythropoiesis.
• Tissue specificityPredominantly expressed in muscle and brain.
• Sequence similaritiesContains 1 RRM (RNA recognition motif) domain.
• Cellular localizationNucleus. Cytoplasm.

Target information above from: UniProt accession Q9NWB1 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 54715 Human
  • Entrez Gene: 268859 Mouse
  • Omim: 605104 Human
  • SwissProt: Q9NWB1 Human
  • SwissProt: Q9JJ43 Mouse
  • Unigene: 459842 Human
  • Unigene: 370334 Mouse

• Alternative names
  A2 BP1 antibodyA2BP 1 antibodyA2BP antibody

  A2BP1 antibodyAtaxin 2 binding protein 1 antibodyAtaxin-2-binding protein 1 antibodyAtaxin2 binding protein 1 antibodyFOX 1 antibodyFox-1 homolog A antibodyFOX1 antibodyHexaribonucleotide binding protein 1 antibodyHexaribonucleotide-binding protein 1 antibodyHRNBP 1 antibodyHRNBP1 antibodyRBFOX1 antibodyRFOX1_HUMAN antibodyRNA binding protein fox-1 homolog 1 antibody

  see all

Anti-A2BP1 / Fox1 antibody images

• 

  Western blot - A2BP1 / Fox1 antibody (ab94581)
  All lanes : Anti-A2BP1 / Fox1 antibody (ab94581) at 1 µg/ml
  
  Lane 1 : Forebrain (Mouse) Tissue Lysate
  Lane 2 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
  developed using the ECL technique
  
  Performed under reducing conditions.
  
  Predicted band size : 43 kDa
  Observed band size : 43 kDa
  Additional bands at : 110 kDa,28 kDa,37 kDa,56 kDa. We are unsure as to the identity of these extra bands.
  
  Exposure time : 2 minutes
• 

  Immunocytochemistry/ Immunofluorescence - A2BP1 / Fox1 antibody (ab94581)

  ICC/IF image of ab94581 stained MCF-7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab94581 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in HeLa, Hek293, and HepG2 cell types at 1ug/ml (4% formaldehyde fixed, 10 min)

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-A2BP1 / Fox1 antibody (ab94581)

  IHC image of A2BP1 / Fox1 staining in Human skeletal muscle formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab94581, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

  For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.