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All lanes : Anti-A2ML1 antibody (ab72872) at 1/500 dilution
Lane 1 : A2ML1 transfected 293T cell lysate
Lane 2 : non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
Predicted band size : 18 kDa
Observed band size : 20 kDa (why is the actual band size different from the predicted?)
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