A431 (Human epithelial carcinoma cell line) Membrane Lysate - tumor cell line (ab30134)

Overview

Properties

  • FormLiquid
  • Storage instructionsStore at -80°C. Allow to warm to room temp and agitate gently before aliquotting.
  • Storage bufferPreservative: None
    Constituents: 2% SDS, 10% Glycerol, 0.1% Bromophenol blue, Protease inhibitor, Sodium chloride, EDTA, Magnesium chloride, HEPES, 0.15M Beta mercaptoethanol
  • Concentration information loading...
  • Lysate notes Protein is isolated from whole tissue homogenates using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70ºC. Quality control: 1. The isolated protein pattern on SDS-PAGE gel is visualized by coomassie blue staining. The pattern is consistent with each lot. 2. The isolated protein is Western Blot analyzed by either a GAPDH or beta-actin antibody. The expression level is consistent with each lot.
  • Research Areas
  • BackgroundA431 cells are a positive control for EGFR as they express high levels of EGFR. They also used for cell cycle studies and can be stimulated with EGF to stimulate EGFR and are therefore a very useful model to study EGFR downstream signalling cascades eg shc, src, MAPK.

Applications

Our Abpromise guarantee covers the use of ab30134 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB Use at an assay dependent dilution. Samples need to boiled for 5 minutes and put on ice before use.

References for A431 (Human epithelial carcinoma cell line) Membrane Lysate - tumor cell line (ab30134)

ab30134 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"