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A431 (Human epithelial carcinoma cell line) Nuclear Lysate - EGF treated (ab14635)

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    2 questions for ab14635

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    Question 1

    Friday 13-April-2012

    Oui d’accord, j’apprécierais un lysat d’une des deux lignées, n’importe laquelle. Ce serait donc un bon contrôle pour stat5 total et phosphorylé?

    ANSWER:

     

    J'ai mis en place l'envoi d'une unité gratuite de lysat de A431 (Human epithelial carcinoma cell line) Nuclear Lysate - EGF treated, référence ab14635. Le numéro de commande est : *****.

    Ce lysat est recommandé comme contrôle positif pour STAT5 phosphorylé et devrait convenir également pour l'anti-STAT5 ab7969.

    J'espère que ce lysat vous sera utile. N'hésitez pas à nous contacter de nouveau si vous avez d'autres questions.

    Question 2

    Wednesday 17-January-2007

    What antibody you do QC in this nuclear lysate?

    ANSWER:

     

    Thank you for your enquiry.

    ab14635 (A431 (Human epithelial carcinoma cell line) Nuclear Lysate - EGF treated) has been quality control tested by Western blot and the Electrophoretic Mobility Shift Assay (EMSA). Positive Transcription Factors in Western: c-Jun, C/EBPb, Sp1, YY1 and in EMSA: Sp1, USF, YY1.

    Please don't hesitate to contact us again if you have further questions.

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