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A431 (Human epithelial carcinoma cell line) Nuclear Lysate - EGF treated (ab14635)

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    Overview

    Product name

    A431 (Human epithelial carcinoma cell line) Nuclear Lysate - EGF treated
    See all A431 products (4) ...

    General notes

    Cells were cultured in medium supplemented with 10 ng/mL EGF (epidermal growth factor) for 10 minutes at 37°C immediately prior to harvesting. We recommend aliquoting the extracts into single use fractions and then storing.

    Properties

    Form

    Liquid

    Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Storage buffer

    Preservative: None
    Constituents: 20% Glycerol, 20mM HEPES, 100mM Potassium Chloride, 1mM Magnesium Chloride, 0.5mM PMSF, 0.5mM DTT, 10 ng/mL EGF (epidermal growth factor). pH 7.9

    Concentration

    Concentration information loading...

    Research areas

    Kits/ Lysates/ Other >> Lysates >> Nuclear Lysates >> Human >> Other

    Background

    A431 cells are a positive control for EGFR as they express high levels of EGFR. They also used for cell cycle studies and can be stimulated with EGF to stimulate EGFR and are therefore a very useful model to study EGFR downstream signalling cascades eg shc, src, MAPK.

    Applications

    Application notes

    Extracts have been quality control tested by Western blot and the Electrophoretic Mobility Shift Assay (EMSA). Positive Transcription Factors in Western: c-Jun, C/EBPb, Sp1, YY1 and in EMSA: Sp1, USF, YY1.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    References for A431 (Human epithelial carcinoma cell line) Nuclear Lysate - EGF treated (ab14635)

    ab14635 has not yet been referenced specifically in any publications.

    Publishing research using ab14635? Please let us know so that we can cite the reference in this datasheet

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"