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A431 (Human epithelial carcinoma cell line) Nuclear Lysate - EGF treated (ab14635)

Q&A (2)

Overview

  • Product nameA431 (Human epithelial carcinoma cell line) Nuclear Lysate - EGF treatedSee all A431 lysates ...
  • General notesCells were cultured in medium supplemented with 10 ng/mL EGF (epidermal growth factor) for 10 minutes at 37°C immediately prior to harvesting. We recommend aliquoting the extracts into single use fractions and then storing.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 20% Glycerol, 20mM HEPES, 100mM Potassium Chloride, 1mM Magnesium Chloride, 0.5mM PMSF, 0.5mM DTT, 10 ng/mL EGF (epidermal growth factor). pH 7.9
  • Concentration information loading...
  • Research Areas
  • BackgroundA431 cells are a positive control for EGFR as they express high levels of EGFR. They also used for cell cycle studies and can be stimulated with EGF to stimulate EGFR and are therefore a very useful model to study EGFR downstream signalling cascades eg shc, src, MAPK.

    • Applications

  • Application notesExtracts have been quality control tested by Western blot and the Electrophoretic Mobility Shift Assay (EMSA). Positive Transcription Factors in Western: c-Jun, C/EBPb, Sp1, YY1 and in EMSA: Sp1, USF, YY1.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • References for A431 (Human epithelial carcinoma cell line) Nuclear Lysate - EGF treated (ab14635)

    ab14635 has not yet been referenced specifically in any publications.

    Publishing research using ab14635 ? Please let us know so that we can cite the reference in this datasheet

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    Submit a Question for ab14635

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    Oui d’accord, j’apprécierais un lysat d’une des deux lignées, n’importe laquelle. Ce serait donc un bon contrôle pour stat5 total et phosphorylé?

    J'ai mis en place l'envoi d'une unité gratuite de lysat de A431 (Human epithelial carcinoma cell line) Nuclear Lysate - EGF treated, référence ab14635. Le numéro de commande est : *****.

    Ce lysat est recommandé...

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    What antibody you do QC in this nuclear lysate?

    Thank you for your enquiry. ab14635 (A431 (Human epithelial carcinoma cell line) Nuclear Lysate - EGF treated) has been quality control tested by Western blot and the Electrophoretic Mobility Shift Assay (EMSA). Positive Transcription Factors in Wester...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"