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Products:Kits/ Lysates/ Other >> Lysates >> Whole Cell Lysates >> Human >> Other
Overview
Product name
A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
See all A431 products (4) ...
General notes
Cell line: A431 (Human epidermoid carcinoma)
Growth media: DMEM & 10% FBS
Tested applications
Properties
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
pH: 6.80
Constituent: 5% Beta mercaptoethanol
Concentration
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Purity
Whole Cell Lysate
Lysate notes
A431 cell lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl flouride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.
Research areas
Kits/ Lysates/ Other >> Lysates >> Whole Cell Lysates >> Human >> Other
Background
A431 cells are a positive control for EGFR as they express high levels of EGFR. They also used for cell cycle studies and can be stimulated with EGF to stimulate EGFR and are therefore a very useful model to study EGFR downstream signalling cascades eg shc, src, MAPK.
Applications
Show applications key
Our Abpromise guarantee covers the use of ab7909 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at an assay dependent concentration. (Ready to load on SDS-PAGE for Western blotting, 20 µg per lane is recommended for mini gel.)
References for A431 (Human epithelial carcinoma cell line) Whole Cell Lysate (ab7909)
ab7909 has not yet been referenced specifically in any publications.
Publishing research using ab7909? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"