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A431 (Human epithelial carcinoma cell line) Whole Cell Lysate (ab7909)

Q&A (5)

Overview

  • Product nameA431 (Human epithelial carcinoma cell line) Whole Cell LysateSee all A431 lysates ...
  • General notesCell line: A431 (Human epidermoid carcinoma) Growth media: DMEM & 10% FBS
  • Tested applicationsWB more details

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferpH: 6.80
    Constituent: 5% Beta mercaptoethanol
  • Concentration information loading...
  • PurityWhole Cell Lysate
  • Lysate notes A431 cell lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl flouride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.
  • Research Areas
  • BackgroundA431 cells are a positive control for EGFR as they express high levels of EGFR. They also used for cell cycle studies and can be stimulated with EGF to stimulate EGFR and are therefore a very useful model to study EGFR downstream signalling cascades eg shc, src, MAPK.

    • Applications

    Our Abpromise guarantee covers the use of ab7909 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    WB WB: Use at an assay dependent concentration. Ready to load on SDS-PAGE for Western blotting, 20 µg per lane is recommended for mini gel.

    References for A431 (Human epithelial carcinoma cell line) Whole Cell Lysate (ab7909)

    ab7909 has not yet been referenced specifically in any publications.

    Publishing research using ab7909 ? Please let us know so that we can cite the reference in this datasheet

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    Submit a Question for ab7909

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    If you do not have ovary cell lysate at present, the human testis tissue lysate will do, too.(http://www.abcam.com/Testis-Human-Tissue-Lysate-adult-normal-tissue-ab30257.html). Thank  you very much!

    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I have issued a free of charge control lysate (ab30257) as goodwill with the order number. To check the s...

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    I have read the two papers you mentioned about, but I still do not think they are very convincing. First, the  GnRH receptor is different from  HCG receptor either in  function or in structure. Even the existence of  GnRH receptor in HepG2 cells does not ...

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    Thank you for taking the time to look into this and for your response.

    We have not tested this in HepG2 cells and the positive control recommendations were based on the literature. I thank you for your recommendation with this and have removed ...

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    Phoned because of concern regarding the positive controls listed on the datasheet. According to published reference, HepG2 is a negative control.

    Thank you for contacting us. I was able to find the reference that you spoke of on the phone (ShanLX, HardyMP Endocrinology.1992 Sep;131(3):1107-14. (PMID:1505454,http://www.ncbi.nlm.nih.gov/pubmed/1505454). During my research for this question I w...

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    What would be a suitable positive control for this product 14-3-3 (phospho S) antibody (0014-33)?

    Thank you for your enquiry. For immunohistochemistry paraffin-embedded sections, human breast carcinoma was used in as control. For Western Blot QC, lysates were prepared from A431 cells, untreated or calyculin A-treated. A431 cells are a human epi...

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    Hi, I am interested to buy the lysate A431+EGF but it is not to do western blot, it is to do some immunoisolation of phosphoproteins. So I don't want the lysate in SDS-Page buffer. I want it in lysis buffer. Do you tell me if you have this kind of lysate ...

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    Thank you for your enquiry and your interest in our products. Unfortunately, we cannot change the constitution of the storage buffer for this product (Ab7909: A431 whole cell lysate). If you cannot find what you are looking for within the Abcam cata...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"