You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Reassurance, Refunds & Replacements

If your product does not perform as described on this datasheet, we will refund or replace your product...

Read our guarantee »
 

A431 (Human epithelial carcinoma cell line) Whole Cell Lysate - tumor cell line (ab30132)

    CodeSizePriceAbpointsAvailability
        
    Add to basket
     
    • -
    •   
    •   
    •   
    •  
    •   
    •   
    Updating...

    Overview

    Product name

    A431 (Human epithelial carcinoma cell line) Whole Cell Lysate - tumor cell line
    See all A431 products (4) ...

    Tested applications

    WBmore details

    Properties

    Form

    Liquid

    Storage instructions

    Store at -80°C. Allow to warm to room temp and agitate gently before aliquotting.

    Storage buffer

    Preservative: None
    Constituents: 2% SDS, 10% Glycerol, 0.1% Bromophenol blue, Protease inhibitor, Sodium chloride, EDTA, Magnesium chloride, HEPES, 0.15M Beta mercaptoethanol

    Concentration

    Concentration information loading...

    Lysate notes

    Protein is isolated from whole tissue homogenates using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70ºC. Quality control: 1. The isolated protein pattern on SDS-PAGE gel is visualized by coomassie blue staining. The pattern is consistent with each lot. 2. The isolated protein is Western Blot analyzed by either a GAPDH or beta-actin antibody. The expression level is consistent with each lot.

    Research areas

    Kits/ Lysates/ Other >> Lysates >> Whole Cell Lysates >> Human >> Other

    Background

    A431 cells are a positive control for EGFR as they express high levels of EGFR. They also used for cell cycle studies and can be stimulated with EGF to stimulate EGFR and are therefore a very useful model to study EGFR downstream signalling cascades eg shc, src, MAPK.

    Applications

    Show applications key

    Our Abpromise guarantee covers the use of ab30132 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • ShowHide

      WB

       WB: Use at an assay depe...Read more →

      WB: Use at an assay dependent dilution. (Samples need to boiled for 5 minutes and put on ice before use.)

    References for A431 (Human epithelial carcinoma cell line) Whole Cell Lysate - tumor cell line (ab30132)

    ab30132 has not yet been referenced specifically in any publications.

    Publishing research using ab30132? Please let us know so that we can cite the reference in this datasheet

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"