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Product Name
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A431 (Human epithelial carcinoma cell line) Whole Cell Lysate - tumor cell line
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Product type
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Lysates
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Description
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A431 (Human epithelial carcinoma cell line) Whole Cell Lysate - tumor cell line
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Application notes
(see key)
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Recommended dilutions
WB: Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Research areas
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Kits/ Lysates/ Other >> Lysates >> Whole Cell Lysates >> Human >> Other
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Relevance
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A431 cells are a positive control for EGFR as they express high levels of EGFR. They also used for cell cycle studies and can be stimulated with EGF to stimulate EGFR and are therefore a very useful model to study EGFR downstream signalling cascades eg shc, src, MAPK.
Protein is isolated from whole tissue homogenates using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70ºC.
Quality control:
1. The isolated protein pattern on SDS-PAGE gel is visualized by coomassie blue staining. The pattern is consistent with each lot.
2. The isolated protein is Western Blot analyzed by either a GAPDH or beta-actin antibody. The expression level is consistent with each lot.
Ethics:
Human tissue samples are collected with the informed consent of the donors & relatives and are excised by licensed medical doctors. Tumor tissue samples are diagnosed and identified by at least two different evaluators. If you have more questions then please feel free to contact us.
Donor information can be supplied on request.
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Storage buffer
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Preservative: None
Constituents: 2% SDS, 10% Glycerol, 0.1% Bromophenol blue, Protease inhibitor, Sodium chloride, EDTA, Magnesium chloride, HEPES, 0.15M Mercaptoethanol
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Form
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Liquid
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Concentration
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3.750 mg/ml
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Storage instructions
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Store at -80°C. Allow to warm to room temp and agitate gently before aliquotting.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this A431 (Human epithelial carcinoma cell line) Whole Cell Lysate - tumor cell line is on this datasheet. But please do contact us if you would like any reassurance! |
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Search PubMed (MEDLINE) for references to A431
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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