Anti-A4GNT antibody [5B5] (ab131649)

Overview

• Product nameAnti-A4GNT antibody [5B5]See all A4GNT primary antibodies ...
• Description
  Mouse monoclonal [5B5] to A4GNT
• Tested applicationsWB, IHC-P more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Recombinant fragment, corresponding to amino acids 121-340 of Human A4GNT produced in E.coli (NP_057245).

• Positive controlHEK293T cells transfected with pCMV6-ENTRY A4GNT cDNA; Human pancreas carcinoma tissue.
• General notesDilute in PBS (pH7,30) before use.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
• Storage bufferpH: 7,30
  Preservative: 0.02% Sodium azide
  Constituents: 48% PBS, 50% Glycerol, 1% BSA
• Concentration information loading...
• PurityProtein G purified
• Purification notesPurified from Mouse ascites fluid by affinity chromatography.
• Clonality Monoclonal
• Clone number5B5
• IsotypeIgG1
• Research Areas
  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Energy transfer pathways
  • Energy Metabolism

  • Signal Transduction
  • Metabolism
  • Energy Metabolism

Applications

Target

• FunctionNecessary for the synthesis of type III mucin. Catalyzes the transfer of N-acetylglucosamine (GlcNAc) to core 2 branched O-glycans.
• Tissue specificityDetected in stomach and pancreas.
• PathwayProtein modification; protein glycosylation.
• Sequence similaritiesBelongs to the glycosyltransferase 32 family.
• DomainThe conserved DXD motif is involved in enzyme activity.
• Cellular localizationGolgi apparatus membrane.

Target information above from: UniProt accession Q9UNA3 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 51146 Human
  • SwissProt: Q9UNA3 Human
  • Unigene: 278960 Human

• Alternative names
  4-N-acetylglucosaminyltransferase antibodyA4GCT_HUMAN antibodyA4GNT antibody

  Alpha 1 4 N acetylglucosaminyltransferase antibodyAlpha-1 antibodyAlpha4GnT antibodyEC 2.4.1. antibodyMGC149493 antibody

  see all

Anti-A4GNT antibody [5B5] images

• 

  Western blot - Anti-A4GNT antibody [5B5] (ab131649)
  All lanes : Anti-A4GNT antibody [5B5] (ab131649)
  
  Lane 1 : HEK293T cells transfected with pCMV6-ENTRY control cDNA
  Lane 2 : HEK293T cells transfected with pCMV6-ENTRY A4GNT cDNA
  
  Lysates/proteins at 5 µg per lane.
  
  
  Predicted band size : 39 kDa
  
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-A4GNT antibody [5B5] (ab131649)
  Immunohistochemical analysis of paraffin-embedded Human pancreas carcinoma tissue labelling A4GNT with ab131649 at 1/150 dilution.