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Western blot - A4GNT antibody (ab75111)
Western blot - A4GNT antibody (ab75111)
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Product Name 

A4GNT antibody

 

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Product type 

Primary antibodies

Description 

Rabbit polyclonal to A4GNT

Immunogen 

Synthetic peptide derived from an internal sequence of human A4GNT.

Reacts with 
(species key)

Hu

Tested applications 
(see key)

ELISA, WB


Abreviews 

Customer reviews
(see key)


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Application notes 
(see key)

Recommended dilutions
ELISA: 1/40000.
WB: 1/500 - 1/1000. Predicted molecular weight: 39 kDa.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Extracts from K562 cells.

Cellular localization 

Golgi apparatus; Single-pass type II membrane protein.

Research areas 

Signal Transduction >> Metabolism >> Energy Metabolism

Relevance 

A4GNT belongs to the glycosyltransferase 32 family. It catalyzes the transfer of N-acetylglucosamine (GlcNAc) to core 2 branched O-glycans and is necessary for the synthesis of type III mucin. It forms a unique glycan, GlcNAcalpha1-->4Galbeta-->R and is largely associated with the Golgi apparatus membrane.

 

A4GNT antibody (ab75111)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

51146    (Human)

GeneCard

    

GC03M139325    (Human)

SwissProt

    

Q9UNA3    (Human)

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this A4GNT antibody is on this datasheet. But please do contact us if you would like any reassurance!


See below for A4GNT antibody images, references, products related to ab75111 and other tools.

 

A4GNT antibody images:

Product image  Western blot - A4GNT antibody (ab75111)

Western blot - A4GNT antibody (ab75111)

All lanes : A4GNT antibody (ab75111) at 1/500 dilution

Lane 1 : Extracts from K562 cells
Lane 2 : Extracts from K562 cells with immunizing peptide at 5 µg

Lysates/proteins at 15 µg per lane.


Predicted band size : 39 kDa
Observed band size : 39 kDa

 
 

Search PubMed (MEDLINE) for references to A4GNT

 

A4GNT antibody - more information

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