|
|
|
|
Western blot - A4GNT antibody (ab75111)

(enlarge)
|
|
|
|
|
Product Name
|
A4GNT antibody
|
| |
See all A4GNT antibodies (2)...
|
|
Product type
|
Primary antibodies
|
|
Description
|
Rabbit polyclonal to A4GNT
|
|
Immunogen
|
Synthetic peptide derived from an internal sequence of human A4GNT.
|
|
Reacts with
(species key)
|
Hu
|
|
Tested applications
(see key)
|
ELISA, WB
|
|
|
Abreviews
|
|
|
|
Application notes
(see key)
|
Recommended dilutions ELISA: 1/40000. WB: 1/500 - 1/1000. Predicted molecular weight: 39 kDa.
Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
|
|
Positive control
(see definition)
|
Extracts from K562 cells.
|
|
Cellular localization
|
Golgi apparatus; Single-pass type II membrane protein.
|
|
Research areas
|
Signal Transduction >> Metabolism >> Energy Metabolism
|
|
Relevance
|
A4GNT belongs to the glycosyltransferase 32 family. It catalyzes the transfer of N-acetylglucosamine (GlcNAc) to core 2 branched O-glycans and is necessary for the synthesis of type III mucin. It forms a unique glycan, GlcNAcalpha1-->4Galbeta-->R and is largely associated with the Golgi apparatus membrane.
|
|
|
|
|
Database links
|
The links below go to external sites and will open in a new browser window
|
|
Raised in
|
Rabbit
|
|
Clonality
|
Polyclonal
|
|
Isotype
|
IgG
|
|
Purity
|
Immunogen affinity purified
|
|
Storage buffer
|
Preservative: 0.02% Sodium Azide Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4 Material safety datasheets (MSDS) for this product: Glycerol MSDS Sodium Azide MSDS
|
|
Form
|
Liquid
|
|
Concentration
|
1.000 mg/ml
|
|
Storage instructions
|
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
|
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this A4GNT antibody is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
|
See below for A4GNT antibody images, references, products related to ab75111 and other tools.
|
| |
A4GNT antibody images:
|
Western blot - A4GNT antibody (ab75111)
All lanes : A4GNT antibody (ab75111) at 1/500 dilution
Lane 1 : Extracts from K562 cells Lane 2 : Extracts from K562 cells with immunizing peptide at 5 µg
Lysates/proteins at 15 µg per lane.
Predicted band size : 39 kDa Observed band size : 39 kDa
|
| |
| |
|
Search PubMed (MEDLINE) for references to A4GNT
|
| |
A4GNT antibody - more information
|
Customer reviews (feedback) regarding A4GNT antibody |
Customer FAQs regarding A4GNT antibody |
Protocols for A4GNT antibody |
Price and availability of products related to A4GNT antibody |
| |
A4GNT antibody - related products:
|
|
|
| |
A4GNT antibody - other tools:
|
Contact Abcam with a Technical Enquiry about A4GNT antibody |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"