Overview
- Product nameAnti-A4GNT antibodySee all A4GNT primary antibodies ...
- DescriptionGoat polyclonal to A4GNT
- Tested applicationsWB more details
- Species reactivityReacts with: Human
Predicted to work with: Cow, Dog - Immunogen
Synthetic peptide: C-PRTYRDLIKGPEGS, corresponding to C terminal amino acids 317-330 of Human A4GNT (NP_057245.1).
- Positive controlK562 cell lysate
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris saline, pH 7.3 -
Concentration information loading... - PurityImmunogen affinity purified
- Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab99187 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 39 kDa). |
Target
- FunctionNecessary for the synthesis of type III mucin. Catalyzes the transfer of N-acetylglucosamine (GlcNAc) to core 2 branched O-glycans.
- Tissue specificityDetected in stomach and pancreas.
- PathwayProtein modification; protein glycosylation.
- Sequence similaritiesBelongs to the glycosyltransferase 32 family.
- DomainThe conserved DXD motif is involved in enzyme activity.
- Cellular localizationGolgi apparatus membrane.
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Database links
- Entrez Gene: 51146 Human
- SwissProt: Q9UNA3 Human
- Unigene: 278960 Human
Target information above from: UniProt accession
Q9UNA3
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- 4-N-acetylglucosaminyltransferase antibodyA4GCT_HUMAN antibodyA4GNT antibody
- Alpha 1 4 N acetylglucosaminyltransferase antibodyAlpha-1 antibodyAlpha4GnT antibodyEC 2.4.1. antibodyMGC149493 antibody
see all
Anti-A4GNT antibody images
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Western blot - A4GNT antibody (ab99187)Anti-A4GNT antibody (ab99187) at 1 µg/ml + K562 cell lysate in RIPA buffer at 35 µg
developed using the ECL technique
Predicted band size : 39 kDa
Observed band size : 40 kDa (why is the actual band size different from the predicted?)
References for Anti-A4GNT antibody (ab99187)
ab99187 has not yet been referenced specifically in any publications.
