Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Kits/ Lysates/ Other >> Lysates >> Whole Cell Lysates >> Human >> Other
Overview
Product name
A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate
General notes
Cell line: A549 (Human lung carcinoma)
Growth media: DMEM and 10% NCS (Newborn calf serum)
Tested applications
Properties
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
pH: 6.80
Constituent: 5% Beta mercaptoethanol
Concentration
Concentration information loading...
Purity
Whole Cell Lysate
Lysate notes
A549 cell lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl flouride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.
Research areas
Kits/ Lysates/ Other >> Lysates >> Whole Cell Lysates >> Human >> Other
Background
A-549 cells are used as an in vitro model for a type II pulmonary epithelial cell model for drug metabolism. Cells are cytokeratin positive by immunoperoxidase staining. This line was initiated in 1972 by D.J. Giard, et al. through explant culture of lung carcinomatous tissue from a 58-year-old Caucasian male (PubMed 4357758). Further studies by M. Lieber, et al. revealed that A549 cells could synthesize lecithin with a high percentage of desaturated fatty acids utilizing the cytidine diphosphocholine pathway (PubMed 175022).
Applications
Show applications key
Our Abpromise guarantee covers the use of ab7910 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at an assay dependent concentration. (A549 cell lysate is ready to load on SDS-PAGE for Western blotting, 20 µg per lane is recommended for mini gel.)
References for A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate (ab7910)
ab7910 has not yet been referenced specifically in any publications.
Publishing research using ab7910? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"