You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Overview

  • Product nameAnti-AADAC antibodySee all AADAC primary antibodies ...
  • Description
    Rabbit polyclonal to AADAC
  • Tested applicationsWB, ELISA more details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Cow, Dog
  • Immunogen

    A region within synthetic peptide: DDNKLRGLPL TYVITCQYDL LRDDGLMYVT RLRNTGVQVT HNHVEDGFHG, corresponding to C terminal amino acids 325-374 of Human AADAC

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive controlFetal liver cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab62176 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB
ELISA
  • Application notesELISA: Use at an assay dependent dilution. Titre using peptide based assay: 1/1562500
    WB: Use at a concentration of 2.5 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 46 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • FunctionArylacetamide deacetylation is an important enzyme activity in the metabolic activation of arylamine substrates to ultimate carcinogens. Displays major serine hydrolase activity in liver microsomes. Hydrolyzes also flutamide, which is an antiandrogen drug used for the treatment of prostate cancer that occasionaly causes severe hepatotoxicity. Displays cellular triglyceride lipase activity in liver. Increases intracellular fatty acids derived from hydrolysis of newly formed triglyceride stores.
    • Tissue specificityMainly expressed in liver, small intestine, colon and adrenal gland.
    • Sequence similaritiesBelongs to the 'GDXG' lipolytic enzyme family.
    • Cellular localizationEndoplasmic reticulum membrane. Microsome membrane.

      • Target information above from: UniProt accession P22760 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
      • Alternative names
          AAAD_HUMAN antibodyAada antibodyAadac antibody
          Arylacetamide deacetylase (esterase) antibodyArylacetamide deacetylase antibodyCES5A1 antibodyDAC antibody
        see all

      Anti-AADAC antibody images

      • Western blot - AADAC antibody (ab62176)
        Western blot - AADAC antibody (ab62176)
        Anti-AADAC antibody (ab62176) at 2.5 µg/ml (in 5% skim milk / PBS buffer) + Fetal Liver cell lysate at 10 µg

        Secondary
        HRP conjugated anti-Rabbit IgG at 1/50000 dilution

        Predicted band size : 46 kDa
        Observed band size : 42 kDa (why is the actual band size different from the predicted?)

      References for Anti-AADAC antibody (ab62176)

      ab62176 has not yet been referenced specifically in any publications.

      Publishing research using ab62176 ? Please let us know so that we can cite the reference in this datasheet

      Product Wall

      Submit an Abreview for ab62176 Submit a Question for ab62176

      There are currently no Abreviews or Questions for ab62176.
      Please use the links above to contact us or submit feedback about this product.

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"