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Anti-AADAC antibody (ab62176)

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Overview

Product name

Anti-AADAC antibody
See all AADAC products (3) ...

Description

Rabbit polyclonal to AADAC

Tested applications

WB, ELISAmore details

Cross reactivity

Reacts with

Human

Predicted to work with

Cow, Dog

Immunogen

A region within synthetic peptide: DDNKLRGLPL TYVITCQYDL LRDDGLMYVT RLRNTGVQVT HNHVEDGFHG, corresponding to C terminal amino acids 325-374 of Human AADAC

DDNKLRGLPL TYVITCQYDL LRDDGLMYVT RLRNTGVQVT HNHVEDGFHG

Run BLAST with BLAST the sequence with ExPASyRun BLAST with BLAST the sequence with NCBI

Positive control

Fetal liver cell lysate.

Properties

Form

Lyophilised:
Reconstitute in 100 µl of distilled water. Final antibody concentration is 1 mg/ml.

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: None
Constituents: 2% Sucrose, PBS

Concentration

Concentration information loading...

Purity

Protein A purified

Clonality

Polyclonal

Isotype

IgG

Research areas

Signal Transduction >> Metabolism >> Drug metabolism
Signal Transduction >> Metabolism >> Energy Metabolism

  • Western blot - AADAC antibody (ab62176)Western blot - AADAC antibody (ab62176) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab62176 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

ELISA: Use at an assay dependent dilution. Titre using peptide based assay: 1/1562500
WB: Use at a concentration of 2.5 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 46 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Relevance

Microsomal arylacetamide deacetylase (AADAC) competes against the activity of cytosolic arylamine N-acetyltransferase, which catalyzes one of the initial biotransformation pathways for arylamine and heterocyclic amine carcinogens. AADAC displays major serine hydrolase activity in liver microsomes. Hydrolyzes also flutamide, which is an antiandrogen drug used for the treatment of prostate cancer that occasionally causes severe hepatotoxicity. Displays cellular triglyceride lipase activity in liver. Increases intracellular fatty acids derived from hydrolysis of newly formed triglyceride stores.

Cellular localization

Endoplasmic reticulum membrane; Microsome membrane; Single-pass type II membrane protein.

Alternative names

  • Aada antibody
  • Arylacetamide deacetylase (esterase) antibody
  • Arylacetamide deacetylase antibody
  • CES5A1 antibody
  • DAC antibody
see all

Database links

Anti-AADAC antibody images:

  Western blot - AADAC antibody (ab62176)

Western blot - AADAC antibody (ab62176)

Anti-AADAC antibody (ab62176) at 2.5 µg/ml (in 5% skim milk / PBS buffer) + Fetal Liver cell lysate at 10 µg

Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution

Predicted band size : 46 kDa
Observed band size : 42 kDa (why is the actual band size different from the predicted?)


Gel concentration: 12%

References for Anti-AADAC antibody (ab62176)

ab62176 has not yet been referenced specifically in any publications.

Publishing research using ab62176? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"