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Western blot - AADAC antibody (ab62176)
(enlarge)
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Product Name
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AADAC antibody
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See all AADAC antibodies (2)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to AADAC
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Immunogen
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A region within synthetic peptide: DDNKLRGLPL TYVITCQYDL LRDDGLMYVT RLRNTGVQVT HNHVEDGFHG, corresponding to C terminal amino acids 325-374 of Human AADAC
BLAST 'DDNKLRGLPL TYVITCQYDL LRDDGLMYVT RLRNTGVQVT HNHVEDGFHG ' with  or 
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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ELISA, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: Use at an assay dependent dilution. Titre using peptide based assay: 1/1562500
WB: Use at a concentration of 2.5 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 46 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Fetal liver cell lysate.
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Cellular localization
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Endoplasmic reticulum membrane; Single-pass type II membrane protein. Microsome membrane; Single-pass type II membrane protein
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Research areas
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Signal Transduction >> Metabolism >> Drug metabolism
Signal Transduction >> Metabolism >> Energy Metabolism
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Relevance
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Microsomal arylacetamide deacetylase (AADAC) competes against the activity of cytosolic arylamine N-acetyltransferase which catalyzes the N-or O-acetylation of various arylamine and heterocyclic amine substrates and is able to bioactivate several known carcinogens. Arylacetamide deacetylation by AADAC is also important in the metabolic activation of some arylamine substrates to carcinogens.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Protein A purified
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Storage buffer
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Preservative: None
Constituents: 2% Sucrose, PBS
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Form
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Lyophilised
Reconstitute in 100 µl of distilled water. Final antibody concentration is 1 mg/ml.
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this AADAC antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for AADAC antibody images, references, products related to ab62176 and other tools.
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AADAC antibody images:
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 Western blot - AADAC antibody (ab62176)
AADAC antibody (ab62176) at 2.5 µg/ml (in 5% skim milk / PBS buffer) + Fetal Liver cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 46 kDa
Observed band size : 42 kDa (why is the actual band size different from the predicted?)
Gel concentration: 12%
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Search PubMed (MEDLINE) for references to AADAC
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AADAC antibody - more information
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Customer reviews (feedback) regarding AADAC antibody |
Customer FAQs regarding AADAC antibody |
Protocols for AADAC antibody |
Price and availability of products related to AADAC antibody |
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AADAC antibody - related products:
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AADAC antibody - other tools:
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Contact Abcam with a Technical Enquiry about AADAC antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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