Overview

  • Product nameAnti-AADAC antibodySee all AADAC primary antibodies ...
  • Description
    Rabbit polyclonal to AADAC
  • Tested applicationsWB, ELISA more details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat, Horse, Cow, Dog
  • Immunogen

    A region within synthetic peptide: DDNKLRGLPL TYVITCQYDL LRDDGLMYVT RLRNTGVQVT HNHVEDGFHG, corresponding to C terminal amino acids 325-374 of Human AADAC

  • Positive control
    • Fetal liver cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab62176 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 46 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/1562500.

Target

  • FunctionArylacetamide deacetylation is an important enzyme activity in the metabolic activation of arylamine substrates to ultimate carcinogens. Displays major serine hydrolase activity in liver microsomes. Hydrolyzes also flutamide, which is an antiandrogen drug used for the treatment of prostate cancer that occasionaly causes severe hepatotoxicity. Displays cellular triglyceride lipase activity in liver. Increases intracellular fatty acids derived from hydrolysis of newly formed triglyceride stores.
  • Tissue specificityMainly expressed in liver, small intestine, colon and adrenal gland.
  • Sequence similaritiesBelongs to the 'GDXG' lipolytic enzyme family.
  • Cellular localizationEndoplasmic reticulum membrane. Microsome membrane.
  • Target information above from: UniProt accession P22760 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • AAAD_HUMAN antibody
    • Aada antibody
    • Aadac antibody
    • Arylacetamide deacetylase (esterase) antibody
    • Arylacetamide deacetylase antibody
    • CES5A1 antibody
    • DAC antibody
    see all

Anti-AADAC antibody images

  • Anti-AADAC antibody (ab62176) at 2.5 µg/ml (in 5% skim milk / PBS buffer) + Fetal Liver cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 46 kDa
    Observed band size : 42 kDa (why is the actual band size different from the predicted?)

References for Anti-AADAC antibody (ab62176)

ab62176 has not yet been referenced specifically in any publications.

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