Anti-AADACL1 antibody (ab111544)

Overview

  • Product nameAnti-AADACL1 antibodySee all AADACL1 primary antibodies ...
  • Description
    Rabbit polyclonal to AADACL1
  • Tested applicationsICC/IF, WB, IHC-Fr, ICC more details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within internal amino acids 224-273 (LQALDFNTPS YQQSMNTPIL PRHVMVRYWL DYFKGNYDFV EAMIVNNHTS) of Mouse AADACL1 (NP_848887).

  • Positive control
    • Mouse kidney lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab111544 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ICC/IF Use at an assay dependent concentration.
WB Use a concentration of 0.2 - 1 µg/ml. Predicted molecular weight: 46 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-Fr Use at an assay dependent concentration.
ICC 1/200.

Target

  • FunctionHydrolyzes 2-acetyl monoalkylglycerol ether, the penultimate precursor of the pathway for de novo synthesis of platelet-activating factor. May be responsible for cholesterol ester hydrolysis in macrophages, thereby contributing to the development of atherosclerosis. Also involved in organ detoxification by hydrolyzing exogenous organophosphorus compounds. May contribute to cancer pathogenesis by promoting tumor cell migration.
  • Tissue specificityExpressed in monocyte-derived macrophages. Up-regulated in invasive melanoma and breast carcinoma cell lines.
  • Sequence similaritiesBelongs to the 'GDXG' lipolytic enzyme family.
  • Post-translational
    modifications
    N-glycosylated.
  • Cellular localizationMembrane. Microsome.
  • Target information above from: UniProt accession Q6PIU2 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • Arylacetamide deacetylase like 1 antibody
    • Arylacetamide deacetylase-like 1 antibody
    • NCEH antibody
    • Nceh1 antibody
    • NCEH1_HUMAN antibody
    • Neutral cholesterol ester hydrolase 1 antibody
    • Neutral cholesterol ester hydrolase antibody
    see all

Anti-AADACL1 antibody images

  • Anti-AADACL1 antibody (ab111544) at 1 µg/ml + Mouse kidney lysate at 10 µg

    Predicted band size : 46 kDa
    Gel concentration: 12%
  • ab114544 staining AADACL1 in cultured human prostate cells by ICC (Immunocytochemistry). Cells were fixed with methanol and blocked with 1% BSA for 24 hours at 4°C. Samples were incubated with primary antibody (1/200 in blocking buffer) for 2 hours at 37°C. A Biotin-conjugated Goat anti-rabbit polyclonal (1/200) was used as the secondary antibody.

    See Abreview

  • ab111544 staining AADACL1 in Human Prostate cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and blocked with 1% BSA + 2% serum for 24 hours at 4°C. Samples were incubated with primary antibody (1/200 in blocking agent) for 2 hours at 37°C. An undiluted FITC-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.

    See Abreview

References for Anti-AADACL1 antibody (ab111544)

ab111544 has not yet been referenced specifically in any publications.

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Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (Prostate tumour)
Specification Prostate tumour
Fixative Methanol
Permeabilization No
Blocking step BSA as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Aug 10 2012

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Prostate)
Specification Prostate
Fixative Paraformaldehyde
Permeabilization No
Blocking step BSA as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Jun 08 2012

Application Immunocytochemistry
Sample Human Cultured Cells (Prostate)
Specification Prostate
Fixative Methanol
Permeabilization No
Blocking step BSA as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
Username

Mr. Olayinka Oloko

Verified customer

Submitted Apr 09 2012

Application Western blot
Sample Human Cell lysate - whole cell (prostate)
Loading amount 25 µg
Specification prostate
Gel Running Conditions Reduced Denaturing (10% gel)
Blocking step Milk as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Mr. Olayinka Oloko

Verified customer

Submitted Dec 07 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"