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Products:Signal Transduction >> Signaling Pathway >> Lipid Signaling >> Other
This fast track antibody is not yet fully characterised. It is subject to these terms and conditions
Overview
Product name
Anti-AADACL1 antibody
See all AADACL1 products (2) ...
Description
Goat polyclonal to AADACL1
This product is a fast track antibody. It has been affinity purified and shows high titre values against the immunizing peptide by ELISA. Read the terms of use »
Cross reactivity
Predicted to work with
Mouse, Cow, Human
Immunogen
Synthetic peptide: C-RTRNSYIKWLDQN, corresponding to C terminal amino acids 395-407 of human AADACL1 (NP_065843.3)
C-RTRNSYIK WLDQN
Positive control
Human brain (Amygdala, Cerebellum, Hippocampus) lysates
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Tumor biomarkers >> Enzymes >> Other
Signal Transduction >> Metabolism >> Lipid metabolism
Signal Transduction >> Signaling Pathway >> Lipid Signaling >> Other
Applications
Application notes
Peptide ELISA: antibody detection limit dilution: 1/1000.
WB: Predicted molecular weight: 46 kDa.
Preliminary experiments gave an approx 26-28 kDa band in human brain (Amygdala, Cerebellum, Hippocampus) lysates after 1 µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 49.1k Da according to NP_065843.3. The 26-28 kDa band was successfully blocked by incubation with the immunizing peptide.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Hydrolyzes 2-acetyl monoalkylglycerol ether, the penultimate precursor of the pathway for de novo synthesis of platelet-activating factor. May be responsible for cholesterol ester hydrolysis in macrophages, thereby contributing to the development of atherosclerosis. Also involved in organ detoxification by hydrolyzing exogenous organophosphorus compounds. May contribute to cancer pathogenesis by promoting tumor cell migration.
Tissue specificity
Expressed in monocyte-derived macrophages. Up-regulated in invasive melanoma and breast carcinoma cell lines.
Sequence similarities
Belongs to the 'GDXG' lipolytic enzyme family.
Post-translational
modifications
N-glycosylated.
Cellular localization
Membrane. Microsome.
Target information above from: UniProt accessionQ6PIU2
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Arylacetamide deacetylase like 1 antibody
- Arylacetamide deacetylase-like 1 antibody
- NCEH antibody
see all
Database links
- Entrez Gene: 57552 Human
- Entrez Gene: 320024 Mouse
- SwissProt: Q6PIU2 Human
- SwissProt: Q8BLF1 Mouse
- Unigene: 444099 Human
- Unigene: 24576 Mouse
References for Anti-AADACL1 antibody (ab77486)
ab77486 has not yet been referenced specifically in any publications.
Publishing research using ab77486? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"