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Rabbit monoclonal [EPR5126(2)] to AAK1
, Flow Cytmore detailsUnsuitable for:
ICC/IF,IHC-P or IP
Synthetic peptide within Human AAK1. The exact sequence is proprietary.
- HepG2 and 293T cell lysates; permeabilized 293T cells.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA
Tissue culture supernatant
Our Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/1000 - 1/10000. Predicted molecular weight: 104 kDa.
||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Is unsuitable for ICC/IF,IHC-P or IP.
Phosphorylates the AP2M1/mu2 subunit of the adaptor protein complex 2 (AP-2). May play a role in regulating aspects of clathrin-mediated endocytosis.
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
Contains 1 protein kinase domain.
Membrane. Cell membrane. Membrane > clathrin-coated pit. Active when found in clathrin-coated pits at the plasma membrane.
Information by UniProt
- AAK 1 antibody
- Aak1 antibody
- AAK1_HUMAN antibody
Western blot - Anti-AAK1 antibody [EPR5126(2)] (ab134971)
All lanes : Anti-AAK1 antibody [EPR5126(2)] (ab134971) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 104 kDa
Flow Cytometry - Anti-AAK1 antibody [EPR5126(2)] (ab134971)
Flow Cytometric analysis of permeabilized 293T cells labelling AAK1 with ab134971 antibody at 1/10 dilution (red) or a rabbit IgG (negative) control (green).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"