Overview

  • Product name
    Anti-AAK1 antibody [EPR5126(2)]
    See all AAK1 primary antibodies
  • Description
    Rabbit monoclonal [EPR5126(2)] to AAK1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC/IF,IHC-P or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human AAK1. The exact sequence is proprietary.

  • Positive control
    • HepG2 and 293T cell lysates; permeabilized 293T cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab134971 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 104 kDa.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC/IF,IHC-P or IP.
  • Target

    • Function
      Phosphorylates the AP2M1/mu2 subunit of the adaptor protein complex 2 (AP-2). May play a role in regulating aspects of clathrin-mediated endocytosis.
    • Sequence similarities
      Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications
      Autophosphorylated.
    • Cellular localization
      Membrane. Cell membrane. Membrane > clathrin-coated pit. Active when found in clathrin-coated pits at the plasma membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • AAK 1 antibody
      • Aak1 antibody
      • AAK1_HUMAN antibody
      • Adaptor associated kinase 1 antibody
      • Adaptor-associated kinase 1 antibody
      • AP2 associated kinase 1 antibody
      • AP2 associated protein kinase 1 antibody
      • AP2-associated protein kinase 1 antibody
      • DKFZp686F03202 antibody
      • DKFZp686K16132 antibody
      • FLJ23712 antibody
      • FLJ25931 antibody
      • FLJ31060 antibody
      • FLJ42882 antibody
      • FLJ45252 antibody
      • KIAA1048 antibody
      • MGC138170 antibody
      • MGC164568 antibody
      • MGC164570 antibody
      see all

    Images

    • All lanes : Anti-AAK1 antibody [EPR5126(2)] (ab134971) at 1/1000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 104 kDa

    • Flow Cytometric analysis of permeabilized 293T cells labelling AAK1 with ab134971 antibody at 1/10 dilution (red) or a rabbit IgG (negative) control (green).

    References

    This product has been referenced in:
    • Bekerman E  et al. Anticancer kinase inhibitors impair intracellular viral trafficking and exert broad-spectrum antiviral effects. J Clin Invest 127:1338-1352 (2017). Read more (PubMed: 28240606) »

    See 1 Publication for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab134971.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up