Overview

  • Product name
  • Description
    Rabbit polyclonal to AAK1
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat, Cow, Human, Pig
  • Immunogen

    Synthetic peptide within Mouse AAK1 aa 278-306 (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q3UHJ0

  • Positive control
    • Mouse bladder tissue lysate, Mouse brain tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab173329 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100 - 1/500. Predicted molecular weight: 104 kDa.

Target

  • Function
    Phosphorylates the AP2M1/mu2 subunit of the adaptor protein complex 2 (AP-2). May play a role in regulating aspects of clathrin-mediated endocytosis.
  • Sequence similarities
    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Autophosphorylated.
  • Cellular localization
    Membrane. Cell membrane. Membrane > clathrin-coated pit. Active when found in clathrin-coated pits at the plasma membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • AAK 1 antibody
    • Aak1 antibody
    • AAK1_HUMAN antibody
    • Adaptor associated kinase 1 antibody
    • Adaptor-associated kinase 1 antibody
    • AP2 associated kinase 1 antibody
    • AP2 associated protein kinase 1 antibody
    • AP2-associated protein kinase 1 antibody
    • DKFZp686F03202 antibody
    • DKFZp686K16132 antibody
    • FLJ23712 antibody
    • FLJ25931 antibody
    • FLJ31060 antibody
    • FLJ42882 antibody
    • FLJ45252 antibody
    • KIAA1048 antibody
    • MGC138170 antibody
    • MGC164568 antibody
    • MGC164570 antibody
    see all

Images

  • Anti-AAK1 antibody (ab173329) at 1/100 dilution + Mouse bladder tissue lysate at 35 µg

    Predicted band size : 104 kDa
  • Immunohistochemical analysis of paraffin embedded Mouse brain tissue labeling AAK1 with ab173329 at 1/10 followed by peroxidase conjugation of the secondary antibody and DAB staining.

References

ab173329 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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