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Western blot - AAK1 antibody (ab59740)
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Product Name
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AAK1 antibody
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See all AAK1 antibodies (3)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to AAK1
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Immunogen
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Synthetic peptide surrounding amino acid 850 of human AAK1.
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Reacts with
(species key)
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Hu, Ms, Rat
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Specificity
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ab59740 recognizes 95kDa AAK1.
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Tested applications
(see key)
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: Use at a concentration of 0.5 - 4 µg/ml. Detects a band of approximately 95 kDa (predicted molecular weight: 94 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Mouse tissue lysate and rat kidney lysate.
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Cellular localization
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Membrane; Peripheral membrane protein.
Note: Active when found in clathrin-coated pits at the plasma membrane.
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Research areas
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Signal Transduction >> Protein Trafficking >> Vesicle Transport >> Regulation
Signal Transduction >> Protein Trafficking >> Vesicle Transport >> Adapters
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
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Relevance
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AAK1 phosphorylates the AP2M1/mu2 subunit of the adaptor protein complex 2 (AP-2). It may play a role in regulating aspects of clathrin-mediated endocytosis.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Protein A purified
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Storage buffer
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Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 30% Glycerol, 0.5% BSA, PBS Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Thimerosal MSDS
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this AAK1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for AAK1 antibody images, references, products related to ab59740 and other tools.
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AAK1 antibody images:
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References for AAK1 antibody (ab59740)
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This product has been used in:
Hu J et al. Allele-specific silencing of mutant huntingtin and ataxin-3 genes by targeting expanded CAG repeats in mRNAs. Nat Biotechnol 27:478-84 (2009). PubMed: 19412185
If you publish research using ab59740 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to AAK1
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AAK1 antibody - more information
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Customer reviews (feedback) regarding AAK1 antibody |
Customer FAQs regarding AAK1 antibody |
Protocols for AAK1 antibody |
Price and availability of products related to AAK1 antibody |
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AAK1 antibody - related products:
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AAK1 antibody - other tools:
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Contact Abcam with a Technical Enquiry about AAK1 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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Western blot - AAK1 antibody (ab59740)