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Products:Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
Overview
Product name
Anti-AAK1 antibody
See all AAK1 products (5) ...
Description
Rabbit polyclonal to AAK1
Specificity
ab59740 recognizes 95kDa AAK1.
Tested applications
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide surrounding amino acid 850 of human AAK1.
Positive control
Mouse tissue lysate and rat kidney lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 30% Glycerol, 0.5% BSA, PBS
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Protein Trafficking >> Vesicle Transport >> Regulation
Signal Transduction >> Protein Trafficking >> Vesicle Transport >> Adapters
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
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Western blot - AAK1 antibody (ab59740)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab59740 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 0.5 - 4 µg/ml.Detects a band of approximately 95 kDa (predicted molecular weight: 94 kDa).
Target
Function
Phosphorylates the AP2M1/mu2 subunit of the adaptor protein complex 2 (AP-2). May play a role in regulating aspects of clathrin-mediated endocytosis.
Sequence similarities
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
Contains 1 protein kinase domain.
Post-translational
modifications
Autophosphorylated.
Cellular localization
Membrane. Cell membrane. Membrane > clathrin-coated pit. Active when found in clathrin-coated pits at the plasma membrane.
Target information above from: UniProt accessionQ2M2I8
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- AAK 1 antibody
- Aak1 antibody
- AAK1_HUMAN antibody
see all
Database links
- Entrez Gene: 22848 Human
- Entrez Gene: 269774 Mouse
- Entrez Gene: 500244 Rat
- SwissProt: Q2M2I8 Human
- SwissProt: Q3UHJ0 Mouse
- SwissProt: P0C1X8 Rat
- Unigene: 468878 Human
- Unigene: 221038 Mouse
see all
Anti-AAK1 antibody images:
Western blot - AAK1 antibody (ab59740)
All lanes : Anti-AAK1 antibody (ab59740) at 0.5 µg/ml
Lane 1 : Mouse tissue lysate
Lane 2 : Rat kidney tissue lysate
Predicted band size : 94 kDa
Observed band size : 95 kDa (why is the actual band size different from the predicted?)
References for Anti-AAK1 antibody (ab59740)
This product has been referenced in:
- Hu Jet al. Allele-specific silencing of mutant huntingtin and ataxin-3 genes by targeting expanded CAG repeats in mRNAs. Nat Biotechnol 27:478-84 (2009).Read more (PubMed: 19412185) »
See 1 publication for this product
Publishing research using ab59740? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"