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Read our guarantee »Products:Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
Anti-AAK1 antibody
See all AAK1 products (5) ...
Rabbit polyclonal to AAK1
This antibody detects endogenous levels of total AAK1 protein.
ELISA, WBmore details
Reacts with
Human
Predicted to work with
Mouse, Rat
A synthetic peptide derived from the internal region of human AAK1
Extracts from COS7 cells
Liquid
Store at -20°C. Stable for 12 months at -20°C
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
Concentration information loading...
Immunogen affinity purified
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Polyclonal
IgG
Signal Transduction >> Protein Trafficking >> Vesicle Transport >> Regulation
Signal Transduction >> Protein Trafficking >> Vesicle Transport >> Adapters
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
Western blot - AAK1 antibody (ab66005)
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Our Abpromise guarantee covers the use of ab66005 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: 1/20000
WB: 1/500 - 1/1000.Detects a band of approximately 94 , 75 kDa (predicted molecular weight: 94 kDa).
Phosphorylates the AP2M1/mu2 subunit of the adaptor protein complex 2 (AP-2). May play a role in regulating aspects of clathrin-mediated endocytosis.
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
Contains 1 protein kinase domain.
Autophosphorylated.
Membrane. Cell membrane. Membrane > clathrin-coated pit. Active when found in clathrin-coated pits at the plasma membrane.
Target information above from: UniProt accessionQ2M2I8
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Western blot - AAK1 antibody (ab66005)

All lanes : Anti-AAK1 antibody (ab66005) at 1/500 dilution
Lane 1 : Extracts from COS7 cells
Lane 2 : Extracts from COS7 cells, plus 5µg immunizing peptide
Lysates/proteins at 5 µg per lane.
Predicted band size : 94 kDa
Observed band size : 94 kDa
Additional bands at : 75 kDa. We are unsure as to the identity of these extra bands.
ab66005 has not yet been referenced specifically in any publications.
Publishing research using ab66005? Please let us know so that we can cite the reference in this datasheet
Concentration of lot no. is
Concentration not available for this lot.
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All lanes : Anti-AAK1 antibody (ab66005) at 1/500 dilution
Lane 1 : Extracts from COS7 cells
Lane 2 : Extracts from COS7 cells, plus 5µg immunizing peptide
Lysates/proteins at 5 µg per lane.
Predicted band size : 94 kDa
Observed band size : 94 kDa
Additional bands at : 75 kDa. We are unsure as to the identity of these extra bands.
0
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