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Products:Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
Overview
Product name
Anti-AAK1 antibody
See all AAK1 products (5) ...
Description
Rabbit polyclonal to AAK1
Specificity
This antibody detects endogenous levels of total AAK1 protein.
Tested applications
ELISA, WBmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat
Immunogen
A synthetic peptide derived from the internal region of human AAK1
Positive control
Extracts from COS7 cells
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Protein Trafficking >> Vesicle Transport >> Regulation
Signal Transduction >> Protein Trafficking >> Vesicle Transport >> Adapters
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
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Western blot - AAK1 antibody (ab66005)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab66005 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
ELISA: 1/20000ELISA: 1/20000
WB: 1/500 - 1/1000.Detects a band of approximately 94 , 75 kDa (predicted molecular weight: 94 kDa).
Target
Function
Phosphorylates the AP2M1/mu2 subunit of the adaptor protein complex 2 (AP-2). May play a role in regulating aspects of clathrin-mediated endocytosis.
Sequence similarities
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
Contains 1 protein kinase domain.
Post-translational
modifications
Autophosphorylated.
Cellular localization
Membrane. Cell membrane. Membrane > clathrin-coated pit. Active when found in clathrin-coated pits at the plasma membrane.
Target information above from: UniProt accessionQ2M2I8
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- AAK 1 antibody
- Aak1 antibody
- AAK1_HUMAN antibody
see all
Database links
- Entrez Gene: 22848 Human
- Entrez Gene: 269774 Mouse
- Entrez Gene: 500244 Rat
- SwissProt: Q2M2I8 Human
- SwissProt: Q3UHJ0 Mouse
- SwissProt: P0C1X8 Rat
- Unigene: 468878 Human
- Unigene: 221038 Mouse
see all
Anti-AAK1 antibody images:
Western blot - AAK1 antibody (ab66005)
All lanes : Anti-AAK1 antibody (ab66005) at 1/500 dilution
Lane 1 : Extracts from COS7 cells
Lane 2 : Extracts from COS7 cells, plus 5µg immunizing peptide
Lysates/proteins at 5 µg per lane.
Predicted band size : 94 kDa
Observed band size : 94 kDa
Additional bands at : 75 kDa. We are unsure as to the identity of these extra bands.
References for Anti-AAK1 antibody (ab66005)
ab66005 has not yet been referenced specifically in any publications.
Publishing research using ab66005? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"