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Western blot - AAK1 antibody (ab66005)
(enlarge)
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Product Name
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AAK1 antibody
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See all AAK1 antibodies (3)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to AAK1
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Immunogen
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A synthetic peptide derived from the internal region of human AAK1
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Reacts with
(species key)
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Hu, Ms, Rat
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Specificity
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This antibody detects endogenous levels of total AAK1 protein.
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Tested applications
(see key)
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ELISA, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/20000.
WB: 1/500 - 1/1000. Detects a band of approximately 94 and 75 kDa (predicted molecular weight: 94 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Extracts from COS7 cells
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Cellular localization
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Membrane; Peripheral membrane protein.
Note: Active when found in clathrin-coated pits at the plasma membrane.
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Research areas
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Signal Transduction >> Protein Trafficking >> Vesicle Transport >> Regulation
Signal Transduction >> Protein Trafficking >> Vesicle Transport >> Adapters
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
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Relevance
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AAK1 phosphorylates the AP2M1/mu2 subunit of the adaptor protein complex 2 (AP-2). It may play a role in regulating aspects of clathrin-mediated endocytosis.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Purification notes
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The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at -20°C. Stable for 12 months at -20°C
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this AAK1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for AAK1 antibody images, references, products related to ab66005 and other tools.
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AAK1 antibody images:
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 Western blot - AAK1 antibody (ab66005)
All lanes : AAK1 antibody (ab66005) at 1/500 dilution
Lane 1 : Extracts from COS7 cells
Lane 2 : Extracts from COS7 cells, plus 5µg immunizing peptide
Lysates/proteins at 5 µg per lane.
Predicted band size : 94 kDa
Observed band size : 94 kDa
Additional bands at : 75 kDa. We are unsure as to the identity of these extra bands.
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Search PubMed (MEDLINE) for references to AAK1
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AAK1 antibody - more information
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Customer reviews (feedback) regarding AAK1 antibody |
Customer FAQs regarding AAK1 antibody |
Protocols for AAK1 antibody |
Price and availability of products related to AAK1 antibody |
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AAK1 antibody - related products:
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AAK1 antibody - other tools:
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Contact Abcam with a Technical Enquiry about AAK1 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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