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Western blot - AAK1 antibody (ab66005)
Western blot - AAK1 antibody (ab66005)
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Product Name 

AAK1 antibody

 

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Product type 

Primary antibodies

Description 

Rabbit polyclonal to AAK1

Immunogen 

A synthetic peptide derived from the internal region of human AAK1

Reacts with 
(species key)

Hu, Ms, Rat

Specificity 

This antibody detects endogenous levels of total AAK1 protein.

Tested applications 
(see key)

ELISA, WB


Abreviews 

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Application notes 
(see key)

Recommended dilutions
ELISA: 1/20000.
WB: 1/500 - 1/1000. Detects a band of approximately 94 and 75 kDa (predicted molecular weight: 94 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Extracts from COS7 cells

Cellular localization 

Membrane; Peripheral membrane protein.
Note: Active when found in clathrin-coated pits at the plasma membrane.

Research areas 

Signal Transduction >> Protein Trafficking >> Vesicle Transport >> Regulation
Signal Transduction >> Protein Trafficking >> Vesicle Transport >> Adapters
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases

Relevance 

AAK1 phosphorylates the AP2M1/mu2 subunit of the adaptor protein complex 2 (AP-2). It may play a role in regulating aspects of clathrin-mediated endocytosis.

 

Alternative names AAK1 antibody (ab66005)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

22848    (Human)
269774    (Mouse)
500244    (Rat)

SwissProt

    

Q2M2I8    (Human)
Q3UHJ0    (Mouse)
P0C1X8    (Rat)

Unigene

    

468878    (Human)

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Purification notes 

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at -20°C. Stable for 12 months at -20°C

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this AAK1 antibody is on this datasheet. But please do contact us if you would like any reassurance!


See below for AAK1 antibody images, references, products related to ab66005 and other tools.

 

AAK1 antibody images:

Product image  Western blot - AAK1 antibody (ab66005)

Western blot - AAK1 antibody (ab66005)

All lanes : AAK1 antibody (ab66005) at 1/500 dilution

Lane 1 : Extracts from COS7 cells
Lane 2 : Extracts from COS7 cells, plus 5µg immunizing peptide

Lysates/proteins at 5 µg per lane.


Predicted band size : 94 kDa
Observed band size : 94 kDa
Additional bands at : 75 kDa. We are unsure as to the identity of these extra bands.

 
 

Search PubMed (MEDLINE) for references to AAK1

 

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Lysates

 

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