Anti-AAK1 antibody (ab77082)
Key features and details
- Rabbit polyclonal to AAK1
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
Overview
-
Product name
Anti-AAK1 antibody
See all AAK1 primary antibodies -
Description
Rabbit polyclonal to AAK1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Synthetic 20 amino acid peptide from near the amino terminus of human AAK1 (NP_055726).
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab77082 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 94 kDa.
|
Notes |
---|
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 94 kDa. |
Target
-
Function
Phosphorylates the AP2M1/mu2 subunit of the adaptor protein complex 2 (AP-2). May play a role in regulating aspects of clathrin-mediated endocytosis. -
Sequence similarities
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
Contains 1 protein kinase domain. -
Post-translational
modificationsAutophosphorylated. -
Cellular localization
Membrane. Cell membrane. Membrane > clathrin-coated pit. Active when found in clathrin-coated pits at the plasma membrane. - Information by UniProt
-
Database links
- Entrez Gene: 269774 Mouse
- SwissProt: Q3UHJ0 Mouse
- Unigene: 221038 Mouse
-
Alternative names
- AAK 1 antibody
- Aak1 antibody
- AAK1_HUMAN antibody
see all
Images
Datasheets and documents
-
SDS download
-
Datasheet download
References (2)
ab77082 has been referenced in 2 publications.
- Loi M et al. MHC Class I Internalization via Autophagy Proteins. Methods Mol Biol 1880:455-477 (2019). PubMed: 30610715
- Ligeon LA et al. Analysis of LC3-Associated Phagocytosis and Antigen Presentation. Methods Mol Biol 1519:145-168 (2017). PubMed: 27815878