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Overview

  • Product nameAARE protein (Tagged-His Tag)See all AARE proteins and peptides ...
  • Protein descriptionRecombinant fragment, corresponding to amino acids 560-732 (173 amino acids) of Human AARE with an N terminal His tag. mol wt: 21 kDa. UniProt ID: P13798.
  • Expression hostE. coli

    • Properties

  • Purification notesPurity level >85%
    This protein was expressed as an N-terminal His-tag fusion protein using Escherichia coli, and purified using Immobilized Metal Ion Affinity Chromatography. In some cases, smaller protein fragments may be present in addition to the intended expression product as a result of premature termination during translation in E. coli and subsequent co-purification via the His-tag. In some cases purified proteins run at a molecular weight different to the theoretically calculated molecular weight. This may be as a result of unequally distributed charges in the amino acid sequence. Alternatively, dimerisation of the expression product can occur under oxygen limitation during expression/cultivation.
  • FormLyophilised:Reconstitute with 78 µl aqua dest.
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
  • Concentration information loading...
  • Sequence notesVKDVQFAVEQVLQEEHFDASHVALMGGSHGGFISCHLIGQ YPETYRACVARNPV INIASMLGSTDIPDWCVVEAGFPF SSDCLPDLSVWAEMLDKSPIRYIPQVKTPL LLMLGQED RRVPFKQGMEYYRALKTRNVPVRLLLYPKSTHALSEVEVE SDSFMN AVLWLRTHLGS
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab91897 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE SDS-PAGE: Use at an assay dependent dilution.

    Protein info

    • Alternative names
        AAREACPHACPH_HUMAN
        Acyl peptide hydrolaseAcyl-peptide hydrolaseAcylamino acid releasing enzymeAcylamino-acid-releasing enzymeAcylaminoacyl peptidaseAcylaminoacyl-peptidaseAPEHAPHN acylaminoacyl peptide hydrolaseOPHOxidized protein hydrolase
      see all
  • FunctionThis enzyme catalyzes the hydrolysis of the N-terminal peptide bond of an N-acetylated peptide to generate an N-acetylated amino acid and a peptide with a free N-terminus. It preferentially cleaves off Ac-Ala, Ac-Met and Ac-Ser.
  • Sequence similaritiesBelongs to the peptidase S9C family.
  • Cellular localizationCytoplasm.

    • Target information above from: UniProt accession P13798 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    AARE protein (Tagged-His Tag) images

    • SDS-PAGE - AARE protein (Tagged-His Tag) (ab91897)
      SDS-PAGE - AARE protein (Tagged-His Tag) (ab91897)
      The image shows an electrophoretic assay performed using an Agilent 5100 ALP. In some images coloured control bands can be seen at 15 kDa (green) and/or 240 kDa (purple). The protein-specific band is blue.

    References for AARE protein (Tagged-His Tag) (ab91897)

    ab91897 has not yet been referenced specifically in any publications.

    Publishing research using ab91897 ? Please let us know so that we can cite the reference in this datasheet

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