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Lane 1 : Anti-AATF antibody (ab39631) at 0.5 µg/ml
Lane 2 : Anti-AATF antibody (ab39631) at 1 µg/ml
Lane 3 : Anti-AATF antibody (ab39631) at 2 µg/ml
Lane 1 : Human small intestine cell lysate
Lane 2 : Human small intestine cell lysate
Lane 3 : Human small intestine cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
anti-rabbit IgG HRP at 1/10000 dilution
Predicted band size : 62 kDa
Observed band size : 70 kDa (why is the actual band size different from the predicted?)
(Lanes read from left to right, 1, 2, 3.)
ab39631 at 10µg/ml staining AATF in small intestine by IHC
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