Overview
- Product nameAnti-AATF antibodySee all AATF primary antibodies ...
- DescriptionRabbit polyclonal to AATF
- Tested applicationsWB, IHC-P, ICC/IF more details
- Species reactivityReacts with: Mouse, Human
- Immunogen
A 12 amino acid synthetic peptide from near the carboxy terminus of human AATF.
- Positive controlHuman small intestine cell lysate.
Properties
- FormLiquid
- Storage instructionsStore at +4°C.
- Storage bufferPreservative: 0.02% Sodium Azide
Constituents: PBS -
Concentration information loading... - PurityImmunogen affinity purified
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab39631 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 62 kDa). |
| IHC-P | IHC-P: Use a concentration of 10 µg/ml. |
| ICC/IF | ICC/IF: Use a concentration of 20 µg/ml. |
Target
- FunctionMay function as a general inhibitor of the histone deacetylase HDAC1. Binding to the pocket region of RB1 may displace HDAC1 from RB1/E2F complexes, leading to activation of E2F target genes and cell cycle progression. Conversely, displacement of HDAC1 from SP1 bound to the CDKN1A promoter leads to increased expression of this CDK inhibitor and blocks cell cycle progression. Also antagonizes PAWR mediated induction of aberrant amyloid peptide production in Alzheimer disease (presenile and senile dementia), although the molecular basis for this phenomenon has not been described to date.
- Tissue specificityUbiquitously expressed. Expressed at high levels in brain, heart, kidney, placenta and thymus.
- Sequence similaritiesBelongs to the AATF family.
- Post-translational
modificationsHyperphosphorylated during the G1/S phase transition. - Cellular localizationNucleus > nucleolus.
-
Database links
- Entrez Gene: 26574 Human
- Entrez Gene: 26574 Human
- Entrez Gene: 26574 Human
- Entrez Gene: 56321 Mouse
- Omim: 608463 Human
- SwissProt: Q9NY61 Human
- SwissProt: Q9NY61 Human
- SwissProt: Q9NY61 Human
- SwissProt: Q3URN5 Mouse
- SwissProt: Q9JKX4 Mouse
- Unigene: 195740 Human
- Unigene: 257482 Mouse
see all
Target information above from: UniProt accession
Q9NY61
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- AATF antibodyAATF_HUMAN antibodyApoptosis antagonizing transcription factor antibody
- Apoptosis-antagonizing transcription factor antibodyCHE1 antibodyDED antibodyProtein AATF antibodyRb binding protein Che 1 antibodyRb-binding protein Che-1 antibody
see all
Anti-AATF antibody images
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Lane 1 : Anti-AATF antibody (ab39631) at 0.5 µg/ml
Lane 2 : Anti-AATF antibody (ab39631) at 1 µg/ml
Lane 3 : Anti-AATF antibody (ab39631) at 2 µg/ml
Lane 1 : Human small intestine cell lysate
Lane 2 : Human small intestine cell lysate
Lane 3 : Human small intestine cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
anti-rabbit IgG HRP at 1/10000 dilution
Predicted band size : 62 kDa
Observed band size : 70 kDa (why is the actual band size different from the predicted?) -
ab39631 at 10µg/ml staining AATF in small intestine by IHC
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Immunofluorescence of AATF in Human Small Intestine cells using ab39631 at 20 ug/ml.
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Immunofluorescence of AATF in Human Small Intestine cells using ab39631 at 20 ug/ml.
References for Anti-AATF antibody (ab39631)
This product has been referenced in:
- Niida H et al. Cooperative functions of Chk1 and Chk2 reduce tumour susceptibility in vivo. EMBO J 29:3558-70 (2010). Read more (PubMed: 20834228) »



