Anti-AATF antibody (ab39631)

Overview

• Product nameAnti-AATF antibodySee all AATF primary antibodies ...
• Description
  Rabbit polyclonal to AATF
• Tested applicationsWB, IHC-P, ICC/IF more details
• Species reactivity
  Reacts with: Mouse, Human
  
• Immunogen

  A 12 amino acid synthetic peptide from near the carboxy terminus of human AATF.

• Positive controlHuman small intestine cell lysate.

Properties

• FormLiquid
• Storage instructionsStore at +4°C.
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: PBS
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Epigenetics and Nuclear Signaling
  • Transcription
  • Other factors

  • Cell Biology
  • Apoptosis
  • Intracellular
  • Survivin / IAPs

Applications

Target

• FunctionMay function as a general inhibitor of the histone deacetylase HDAC1. Binding to the pocket region of RB1 may displace HDAC1 from RB1/E2F complexes, leading to activation of E2F target genes and cell cycle progression. Conversely, displacement of HDAC1 from SP1 bound to the CDKN1A promoter leads to increased expression of this CDK inhibitor and blocks cell cycle progression. Also antagonizes PAWR mediated induction of aberrant amyloid peptide production in Alzheimer disease (presenile and senile dementia), although the molecular basis for this phenomenon has not been described to date.
• Tissue specificityUbiquitously expressed. Expressed at high levels in brain, heart, kidney, placenta and thymus.
• Sequence similaritiesBelongs to the AATF family.
• Post-translational
  modificationsHyperphosphorylated during the G1/S phase transition.
• Cellular localizationNucleus > nucleolus.

Target information above from: UniProt accession Q9NY61 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 26574 Human
  • Entrez Gene: 26574 Human
  • Entrez Gene: 26574 Human
  • Entrez Gene: 56321 Mouse
  • Omim: 608463 Human
  • SwissProt: Q9NY61 Human
  • SwissProt: Q9NY61 Human
  • SwissProt: Q9NY61 Human

  • SwissProt: Q3URN5 Mouse
  • SwissProt: Q9JKX4 Mouse
  • Unigene: 195740 Human
  • Unigene: 257482 Mouse

  see all

• Alternative names
  AATF antibodyAATF_HUMAN antibodyApoptosis antagonizing transcription factor antibody

  Apoptosis-antagonizing transcription factor antibodyCHE1 antibodyDED antibodyProtein AATF antibodyRb binding protein Che 1 antibodyRb-binding protein Che-1 antibody

  see all

Anti-AATF antibody images

• 

  Western blot - AATF antibody (ab39631)
  Lane 1 : Anti-AATF antibody (ab39631) at 0.5 µg/ml
  Lane 2 : Anti-AATF antibody (ab39631) at 1 µg/ml
  Lane 3 : Anti-AATF antibody (ab39631) at 2 µg/ml
  
  Lane 1 : Human small intestine cell lysate
  Lane 2 : Human small intestine cell lysate
  Lane 3 : Human small intestine cell lysate
  
  Lysates/proteins at 15 µg per lane.
  
  Secondary
  anti-rabbit IgG HRP at 1/10000 dilution
  
  Predicted band size : 62 kDa
  Observed band size : 70 kDa (why is the actual band size different from the predicted?)
  
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - AATF antibody (ab39631)

  ab39631 at 10µg/ml staining AATF in small intestine by IHC

• 

  Immunocytochemistry/ Immunofluorescence-Anti-AATF antibody(ab39631)
  Immunofluorescence of AATF in Human Small Intestine cells using ab39631 at 20 ug/ml.
  
  
• 

  Immunocytochemistry/ Immunofluorescence-Anti-AATF antibody(ab39631)
  Immunofluorescence of AATF in Human Small Intestine cells using ab39631 at 20 ug/ml.