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Read our guarantee »Products:Cell Biology >> Apoptosis >> Intracellular >> Survivin / IAPs
Anti-AATF antibody
See all AATF products (5) ...
Rabbit polyclonal to AATF
WB, IHC-Pmore details
Reacts with
Mouse, Human
A 12 amino acid synthetic peptide from near the carboxy terminus of human AATF.
Human small intestine cell lysate.
Liquid
Store at +4°C.
Preservative: 0.02% Sodium Azide
Constituents: PBS
Concentration information loading...
Immunogen affinity purified
Polyclonal
IgG
Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Cell Biology >> Apoptosis >> Intracellular >> Survivin / IAPs
Our Abpromise guarantee covers the use of ab39631 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 0.5 - 1 µg/ml.Detects a band of approximately 70 kDa (predicted molecular weight: 62 kDa).
IHC-P: Use a concentration of 10 µg/ml
May function as a general inhibitor of the histone deacetylase HDAC1. Binding to the pocket region of RB1 may displace HDAC1 from RB1/E2F complexes, leading to activation of E2F target genes and cell cycle progression. Conversely, displacement of HDAC1 from SP1 bound to the CDKN1A promoter leads to increased expression of this CDK inhibitor and blocks cell cycle progression. Also antagonizes PAWR mediated induction of aberrant amyloid peptide production in Alzheimer disease (presenile and senile dementia), although the molecular basis for this phenomenon has not been described to date.
Ubiquitously expressed. Expressed at high levels in brain, heart, kidney, placenta and thymus.
Belongs to the AATF family.
Hyperphosphorylated during the G1/S phase transition.
Nucleus > nucleolus.
Target information above from: UniProt accessionQ9NY61
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Western blot - AATF antibody (ab39631)

Lane 1 : Anti-AATF antibody (ab39631) at 0.5 µg/ml
Lane 2 : Anti-AATF antibody (ab39631) at 1 µg/ml
Lane 3 : Anti-AATF antibody (ab39631) at 2 µg/ml
Lane 1 : Human small intestine cell lysate
Lane 2 : Human small intestine cell lysate
Lane 3 : Human small intestine cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
anti-rabbit IgG HRP at 1/10000 dilution
Predicted band size : 62 kDa
Observed band size : 70 kDa (why is the actual band size different from the predicted?)
(Lanes read from left to right, 1, 2, 3.)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - AATF antibody (ab39631)

ab39631 at 10µg/ml staining AATF in small intestine by IHC
This product has been referenced in:
See 1 publication for this product
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Lane 1 : Anti-AATF antibody (ab39631) at 0.5 µg/ml
Lane 2 : Anti-AATF antibody (ab39631) at 1 µg/ml
Lane 3 : Anti-AATF antibody (ab39631) at 2 µg/ml
Lane 1 : Human small intestine cell lysate
Lane 2 : Human small intestine cell lysate
Lane 3 : Human small intestine cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
anti-rabbit IgG HRP at 1/10000 dilution
Predicted band size : 62 kDa
Observed band size : 70 kDa (why is the actual band size different from the predicted?)
(Lanes read from left to right, 1, 2, 3.)

ab39631 at 10µg/ml staining AATF in small intestine by IHC
0
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