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Products:Cell Biology >> Apoptosis >> Intracellular >> Survivin / IAPs
Overview
Product name
Anti-AATF antibody
See all AATF products (5) ...
Description
Rabbit polyclonal to AATF
Tested applications
WB, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
A 12 amino acid synthetic peptide from near the carboxy terminus of human AATF.
Positive control
Human small intestine cell lysate.
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Cell Biology >> Apoptosis >> Intracellular >> Survivin / IAPs
Applications
Show applications key
Our Abpromise guarantee covers the use of ab39631 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 0.5 - 1 µg/ml.Detects a band of approximately 70 kDa (predicted molecular weight: 62 kDa).
IHC-P: Use a concentration of 10 µg/ml
Target
Function
May function as a general inhibitor of the histone deacetylase HDAC1. Binding to the pocket region of RB1 may displace HDAC1 from RB1/E2F complexes, leading to activation of E2F target genes and cell cycle progression. Conversely, displacement of HDAC1 from SP1 bound to the CDKN1A promoter leads to increased expression of this CDK inhibitor and blocks cell cycle progression. Also antagonizes PAWR mediated induction of aberrant amyloid peptide production in Alzheimer disease (presenile and senile dementia), although the molecular basis for this phenomenon has not been described to date.
Tissue specificity
Ubiquitously expressed. Expressed at high levels in brain, heart, kidney, placenta and thymus.
Sequence similarities
Belongs to the AATF family.
Post-translational
modifications
Hyperphosphorylated during the G1/S phase transition.
Cellular localization
Nucleus > nucleolus.
Target information above from: UniProt accessionQ9NY61
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- AATF antibody
- AATF_HUMAN antibody
- Apoptosis antagonizing transcription factor antibody
see all
Database links
- Entrez Gene: 26574 Human
- Entrez Gene: 56321 Mouse
- Omim: 608463 Human
- SwissProt: Q9NY61 Human
- SwissProt: Q3URN5 Mouse
- SwissProt: Q9JKX4 Mouse
- Unigene: 195740 Human
- Unigene: 257482 Mouse
Anti-AATF antibody images:
Western blot - AATF antibody (ab39631)
Lane 1 : Anti-AATF antibody (ab39631) at 0.5 µg/ml
Lane 2 : Anti-AATF antibody (ab39631) at 1 µg/ml
Lane 3 : Anti-AATF antibody (ab39631) at 2 µg/ml
Lane 1 : Human small intestine cell lysate
Lane 2 : Human small intestine cell lysate
Lane 3 : Human small intestine cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
anti-rabbit IgG HRP at 1/10000 dilution
Predicted band size : 62 kDa
Observed band size : 70 kDa (why is the actual band size different from the predicted?)
(Lanes read from left to right, 1, 2, 3.)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - AATF antibody (ab39631)
ab39631 at 10µg/ml staining AATF in small intestine by IHC
References for Anti-AATF antibody (ab39631)
This product has been referenced in:
- Niida Het al. Cooperative functions of Chk1 and Chk2 reduce tumour susceptibility in vivo. EMBO J 29:3558-70 (2010).Read more (PubMed: 20834228) »
See 1 publication for this product
Publishing research using ab39631? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"