|
|
|
|
Western blot - AATF antibody (ab39631)
(enlarge)
|
|
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - AATF antibody (ab39631)
(enlarge)
|
|
|
|
|
Product Name
|
AATF antibody
|
| |
See all AATF antibodies (5)...
|
|
Product type
|
Primary antibodies
|
|
Description
|
Rabbit polyclonal to AATF
|
|
Immunogen
|
A 12 amino acid synthetic peptide from near the carboxy terminus of human AATF.
|
|
Reacts with
(species key)
|
Hu, Ms
|
|
Tested applications
(see key)
|
IHC-P, WB
|
|
|
Abreviews
|
|
|
|
Application notes
(see key)
|
Recommended dilutions
IHC-P: Use at a concentration of 10 µg/ml.
WB: Use at a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 62 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
|
|
Positive control
(see definition)
|
Human small intestine cell lysate.
|
|
Cellular localization
|
Nucleus; nucleolus.
|
|
Research areas
|
Chromatin and Nuclear Signaling >> Transcription >> Other factors
Cell Biology >> Apoptosis >> Intracellular >> Survivin / IAPs
|
|
Relevance
|
AATF (Apoptosis-antagonizing transcription factor), also termed Che-1, was identified as interacting protein of Dlk/ZIP kinase and RNA polymerase II. AATF has additionally been shown to bind Rb, thereby activating transcription factor E2F and promoting cell cycle progression. AATF enhances steroid receptor-mediated transactivation in a hormone and dose-dependent manner. It is involved in the activation of anti-apoptotic pathways.
AATF contains an extremely acidic domain, a putative leucine zipper and a nuclear localisation signal, features which are characteristic of transcription factors. Recently AATF has been shown to protect neural cells against oxidative damage suggesting it may have therapeutic uses in Alzheimer's Disease.
|
|
|
|
|
Database links
|
The links below go to external sites and will open in a new browser window |
|
Raised in
|
Rabbit
|
|
Clonality
|
Polyclonal
|
|
Isotype
|
IgG
|
|
Purity
|
Immunogen affinity purified
|
|
Storage buffer
|
Preservative: 0.02% Sodium Azide
Constituents: PBS Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
|
|
Form
|
Liquid
|
|
Concentration
|
1.000 mg/ml
|
|
Storage instructions
|
Store at +4°C.
|
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this AATF antibody is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
|
See below for AATF antibody images, references, products related to ab39631 and other tools.
|
| |
AATF antibody images:
|
 Western blot - AATF antibody (ab39631)
Lane 1 : AATF antibody (ab39631) at 0.5 µg/ml
Lane 2 : AATF antibody (ab39631) at 1 µg/ml
Lane 3 : AATF antibody (ab39631) at 2 µg/ml
Lane 1 : Human small intestine cell lysate
Lane 2 : Human small intestine cell lysate
Lane 3 : Human small intestine cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
anti-rabbit IgG HRP at 1/10000 dilution
Predicted band size : 62 kDa
Observed band size : 70 kDa (why is the actual band size different from the predicted?)
(Lanes read from left to right, 1, 2, 3.)
|
| |
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - AATF antibody (ab39631)
ab39631 at 10µg/ml staining AATF in small intestine by IHC
|
| |
| |
|
Search PubMed (MEDLINE) for references to AATF
|
| |
AATF antibody - more information
|
Customer reviews (feedback) regarding AATF antibody |
Customer FAQs regarding AATF antibody |
Protocols for AATF antibody |
Price and availability of products related to AATF antibody |
| |
AATF antibody - related products:
|
|
|
| |
AATF antibody - other tools:
|
Contact Abcam with a Technical Enquiry about AATF antibody |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
