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Western blot - AATF antibody (ab65248)
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Immunocytochemistry/ Immunofluorescence-AATF antibody(ab65248)
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Product Name
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AATF antibody
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See all AATF antibodies (5)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to AATF
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Immunogen
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Synthetic peptide derived from N terminus of human AATF.
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Reacts with
(species key)
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Hu, Ms, Rat
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Specificity
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ab65248 detects endogenous levels of total AATF protein.
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Tested applications
(see key)
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ELISA, ICC/IF, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/10000.
ICC/IF: Use at a concentration of 1 µg/ml.
WB: 1/500 - 1/1000. Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Extracts from HeLa cells, HepG2 cells, COLO205 cells and HUVEC cells.
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Cellular localization
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Nucleus; nucleolus.
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Research areas
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Chromatin and Nuclear Signaling >> Transcription >> Other factors
Cell Biology >> Apoptosis >> Intracellular >> Survivin / IAPs
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Relevance
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AATF (Apoptosis-antagonizing transcription factor), also termed Che-1, was identified as interacting protein of Dlk/ZIP kinase and RNA polymerase II. AATF has additionally been shown to bind Rb, thereby activating transcription factor E2F and promoting cell cycle progression. AATF enhances steroid receptor-mediated transactivation in a hormone and dose-dependent manner. It is involved in the activation of anti-apoptotic pathways.
AATF contains an extremely acidic domain, a putative leucine zipper and a nuclear localisation signal, features which are characteristic of transcription factors. Recently AATF has been shown to protect neural cells against oxidative damage suggesting it may have therapeutic uses in Alzheimer's Disease.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at -20°C. Stable for 12 months at -20°C
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this AATF antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for AATF antibody images, references, products related to ab65248 and other tools.
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AATF antibody images:
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 Western blot - AATF antibody (ab65248)
All lanes : AATF antibody (ab65248) at 1/500 dilution
Lane 1 : HeLa cell extract
Lane 2 : HepG2 cell extract
Lane 3 : COLO205 cell extract
Lane 4 : HUVEC cell extract
Lane 5 : HUVEC cell extract with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size : 62 kDa
Observed band size : 62 kDa
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Immunocytochemistry/ Immunofluorescence-AATF antibody(ab65248)
ICC/IF image of ab65248 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65248, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Search PubMed (MEDLINE) for references to AATF
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AATF antibody - more information
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Customer reviews (feedback) regarding AATF antibody |
Customer FAQs regarding AATF antibody |
Protocols for AATF antibody |
Price and availability of products related to AATF antibody |
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AATF antibody - related products:
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AATF antibody - other tools:
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Contact Abcam with a Technical Enquiry about AATF antibody |
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