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Products:Cell Biology >> Apoptosis >> Intracellular >> Survivin / IAPs
Overview
Product name
Anti-AATF antibody
See all AATF products (5) ...
Description
Rabbit polyclonal to AATF
Specificity
ab65248 detects endogenous levels of total AATF protein.
Tested applications
ICC/IF, ELISA, WBmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat
Immunogen
Synthetic peptide derived from N terminus of human AATF.
Positive control
Extracts from HeLa cells, HepG2 cells, COLO205 cells and HUVEC cells.
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Cell Biology >> Apoptosis >> Intracellular >> Survivin / IAPs
Applications
Show applications key
Our Abpromise guarantee covers the use of ab65248 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use a concentration of 1 µg/ml
ELISA
ELISA: 1/10000ELISA: 1/10000
WB: 1/500 - 1/1000.Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa).
Target
Function
May function as a general inhibitor of the histone deacetylase HDAC1. Binding to the pocket region of RB1 may displace HDAC1 from RB1/E2F complexes, leading to activation of E2F target genes and cell cycle progression. Conversely, displacement of HDAC1 from SP1 bound to the CDKN1A promoter leads to increased expression of this CDK inhibitor and blocks cell cycle progression. Also antagonizes PAWR mediated induction of aberrant amyloid peptide production in Alzheimer disease (presenile and senile dementia), although the molecular basis for this phenomenon has not been described to date.
Tissue specificity
Ubiquitously expressed. Expressed at high levels in brain, heart, kidney, placenta and thymus.
Sequence similarities
Belongs to the AATF family.
Post-translational
modifications
Hyperphosphorylated during the G1/S phase transition.
Cellular localization
Nucleus > nucleolus.
Target information above from: UniProt accessionQ9NY61
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- AATF antibody
- AATF_HUMAN antibody
- Apoptosis antagonizing transcription factor antibody
see all
Database links
- Entrez Gene: 26574 Human
- Entrez Gene: 56321 Mouse
- Entrez Gene: 114512 Rat
- Omim: 608463 Human
- SwissProt: Q9NY61 Human
- SwissProt: Q3URN5 Mouse
- SwissProt: Q9JKX4 Mouse
- SwissProt: Q9QYW0 Rat
see all
Anti-AATF antibody images:
Western blot - AATF antibody (ab65248)
All lanes : Anti-AATF antibody (ab65248) at 1/500 dilution
Lane 1 : HeLa cell extract
Lane 2 : HepG2 cell extract
Lane 3 : COLO205 cell extract
Lane 4 : HUVEC cell extract
Lane 5 : HUVEC cell extract with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size : 62 kDa
Observed band size : 62 kDa
Immunocytochemistry/ Immunofluorescence-AATF antibody(ab65248)
ICC/IF image of ab65248 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65248, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-AATF antibody (ab65248)
ab65248 has not yet been referenced specifically in any publications.
Publishing research using ab65248? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"