Anti-AATF antibody (ab72148)

Overview

• Product nameAnti-AATF antibodySee all AATF primary antibodies ...
• Description
  Rabbit polyclonal to AATF
• Tested applicationsWB, IP more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Synthetic peptide corresponding to a region between residues 125 and 175 of human AATF (NP_036270.1).

• Positive controlHeLa whole cell lysate.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPreservative: 0.09% Sodium Azide
  Constituents: 0.1% BSA, Tris buffered saline
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Epigenetics and Nuclear Signaling
  • Transcription
  • Other factors

  • Cell Biology
  • Apoptosis
  • Intracellular
  • Survivin / IAPs

Applications

Target

• FunctionMay function as a general inhibitor of the histone deacetylase HDAC1. Binding to the pocket region of RB1 may displace HDAC1 from RB1/E2F complexes, leading to activation of E2F target genes and cell cycle progression. Conversely, displacement of HDAC1 from SP1 bound to the CDKN1A promoter leads to increased expression of this CDK inhibitor and blocks cell cycle progression. Also antagonizes PAWR mediated induction of aberrant amyloid peptide production in Alzheimer disease (presenile and senile dementia), although the molecular basis for this phenomenon has not been described to date.
• Tissue specificityUbiquitously expressed. Expressed at high levels in brain, heart, kidney, placenta and thymus.
• Sequence similaritiesBelongs to the AATF family.
• Post-translational
  modificationsHyperphosphorylated during the G1/S phase transition.
• Cellular localizationNucleus > nucleolus.

Target information above from: UniProt accession Q9NY61 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 26574 Human
  • Entrez Gene: 26574 Human
  • Entrez Gene: 26574 Human
  • Omim: 608463 Human
  • SwissProt: Q9NY61 Human
  • SwissProt: Q9NY61 Human
  • SwissProt: Q9NY61 Human
  • Unigene: 195740 Human

• Alternative names
  AATF antibodyAATF_HUMAN antibodyApoptosis antagonizing transcription factor antibody

  Apoptosis-antagonizing transcription factor antibodyCHE1 antibodyDED antibodyProtein AATF antibodyRb binding protein Che 1 antibodyRb-binding protein Che-1 antibody

  see all

Anti-AATF antibody images

• 

  Western blot - AATF antibody (ab72148)
  All lanes : Anti-AATF antibody (ab72148) at 0.04 µg/ml
  
  Lane 1 : HeLa whole cell lysate at 50 µg
  Lane 2 : HeLa whole cell lysate at 15 µg
  Lane 3 : HeLa whole cell lysate at 5 µg
  
  
  Predicted band size : 62 kDa
  Observed band size : 80 kDa (why is the actual band size different from the predicted?)
  
• 

  Immunoprecipitation - AATF antibody (ab72148)
  Detection of Human AATF by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab72148 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection.