Overview

  • Product name
    Anti-ABAT antibody [EPR4433]
    See all ABAT primary antibodies
  • Description
    Rabbit monoclonal [EPR4433] to ABAT
  • Host species
    Rabbit
  • Specificity
    Mouse cross reactivity tested by western blot and IHC. Rat cross reactivity tested by western blot only.
  • Tested applications
    Suitable for: WB, IP, IHC-Pmore details
    Unsuitable for: Flow Cyt or ICC/IF
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human ABAT aa 1-100. The exact sequence is proprietary.
    (Peptide available as ab202287)

  • Positive control
    • HepG2 cell lysate, SH-SY5Y cell lysae, Human fetal liver tissue lysate, rat brain tissue lysate, rat kidney tissue lysate, mouse brain tissue lysate, Human liver tissue.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab108249 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 56 kDa.
IP 1/10 - 1/100.
IHC-P 1/1000.

See IHC antigen retrieval protocols.

Antigen retrieval is recommended

For unpurified use at 1/250 - 1/500 dilution. 

  • Application notes
    Is unsuitable for Flow Cyt or ICC/IF.
  • Target

    • Function
      Catalyzes the conversion of gamma-aminobutyrate and L-beta-aminoisobutyrate to succinate semialdehyde and methylmalonate semialdehyde, respectively. Can also convert delta-aminovalerate and beta-alanine.
    • Tissue specificity
      Liver > pancreas > brain > kidney > heart > placenta.
    • Involvement in disease
      Defects in ABAT are a cause of gamma-aminobutyrate transaminase deficiency (GABA-AT deficiency) [MIM:613163]. The phenotype of this deficiency includes psychomotor retardation, hypotonia, hyperreflexia, lethargy, refractory seizures, and EEG abnormalities.
    • Sequence similarities
      Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family.
    • Cellular localization
      Mitochondrion matrix.
    • Information by UniProt
    • Database links
    • Alternative names
      • (S) 3 amino 2 methylpropionate transaminase antibody
      • (S)-3-amino-2-methylpropionate transaminase antibody
      • 4 aminobutyrate aminotransferase antibody
      • 4 aminobutyrate aminotransferase, mitochondrial antibody
      • 4-aminobutyrate aminotransferase antibody
      • ABAT antibody
      • FLJ17813 antibody
      • FLJ30272 antibody
      • GABA aminotransferase antibody
      • GABA AT antibody
      • GABA T antibody
      • GABA transaminase antibody
      • GABA transferase antibody
      • GABA-AT antibody
      • GABA-T antibody
      • GABAT antibody
      • GABT_HUMAN antibody
      • Gamma amino N butyrate transaminase antibody
      • Gamma-amino-N-butyrate transaminase antibody
      • hCG1984265 antibody
      • L AIBAT antibody
      • L-AIBAT antibody
      • LAIBAT antibody
      • mitochondrial antibody
      • NPD009 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat liver tissue sections labeling ABAT with Purified ab108249 at 1:1000 dilution (0.9 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)

    • All lanes : Anti-ABAT antibody [EPR4433] (ab108249) at 1/10000 dilution (Purified)

      Lane 1 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates with 5% NFDM /TBST
      Lane 2 : SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysates with 5% NFDM /TBST
      Lane 3 : Mouse brain lysates with 5% NFDM /TBST
      Lane 4 : Rat brain lysates with 5% NFDM /TBST

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

      Predicted band size: 56 kDa
      Observed band size: 50 kDa (why is the actual band size different from the predicted?)

    • ab108249 (purified) at 1:40 dilution (2µg) immunoprecipitating ABAT in HepG2 whole cell lysate.
      Lane 1 (input): HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate 10µg
      Lane 2 (+): ab108249 & HepG2 whole cell lysate
      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab108249 in HepG2 whole cell lysate
      For western blotting, VeriBlot for IP secondary antibody (HRP) (ab131366) was used as the secondary antibody at 1:1000 dilution.
      Blocking and diluting buffer: 5% NFDM/TBST.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse cerebrum tissue sections labeling ABAT with Purified ab108249 at 1:1000 dilution (0.9 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human liver tissue sections labeling ABAT with Purified ab108249 at 1:1000 dilution (0.9 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)

    • Unpurified ab108249, at 1/250 dilution, staining ABAT in Human liver tissue by immunohistochemistry.

    • All lanes : Anti-ABAT antibody [EPR4433] (ab108249) (unpurified)

      Lane 1 : HepG2 cell lysate
      Lane 2 : SH-SY5Y cell lysate
      Lane 3 : Human fetal liver tissue lysate
      Lane 4 : rat brain tissue lysate
      Lane 5 : rat kidney tissue lysate
      Lane 6 : mouse brain tissue lysate

      Predicted band size: 56 kDa

    References

    This product has been referenced in:
    • Chen L  et al. Involvement of upregulation of miR-210 in a rat epilepsy model. Neuropsychiatr Dis Treat 12:1731-7 (2016). WB . Read more (PubMed: 27471387) »
    • De Ita-Pérez D  et al. Synchronization by food access modifies the daily variations in expression and activity of liver GABA transaminase. Biomed Res Int 2014:590581 (2014). WB ; Rat . Read more (PubMed: 24809054) »

    See all 3 Publications for this product

    Customer reviews and Q&As

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Western blot
    Sample
    Sheep Tissue lysate - whole (Liver tissue)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    20 µg
    Specification
    Liver tissue
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Feb 14 2018

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (breast cancer)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    20 µg
    Specification
    breast cancer
    Blocking step
    Milk as blocking agent for 1 hour(s) and 23 minute(s) · Concentration: 0.5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Dec 05 2016

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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