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Western blot - ABAT antibody (ab72765)
Western blot - ABAT antibody (ab72765)
(enlarge)


Western blot - ABAT antibody (ab72765)
Western blot - ABAT antibody (ab72765)
(enlarge)


Product Name 

ABAT antibody

 

See all ABAT antibodies (2)...

Product type 

Primary antibodies

Description 

Mouse polyclonal to ABAT

Immunogen 

Full length human ABAT protein (NP_000654.2)

Reacts with 
(species key)

Hu

Tested applications 
(see key)

WB


Abreviews 

Customer reviews
(see key)


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Application notes 
(see key)

Recommended dilutions
WB: 1/500 - 1/1000. Predicted molecular weight: 56 kDa.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Human liver lysate; lysate from ABAT transfected 293T cells.

Cellular localization 

Mitochondrial matrix

Research areas 

Cancer >> Cancer Metabolism >> Response to hypoxia
Signal Transduction >> Metabolism >> Mitochondrial
Neuroscience >> Neurotransmitter >> Amino Acids >> GABA

Relevance 

4 aminobutyrate aminotransferase (ABAT) is responsible for catabolism of gamma aminobutyric acid (GABA), an important, mostly inhibitory neurotransmitter in the central nervous system, into succinic semialdehyde. The active enzyme is a homodimer of 50 kD subunits complexed to pyridoxal-5-phosphate. The protein sequence is over 95% similar to the pig protein. GABA is estimated to be present in nearly one third of human synapses. ABAT in liver and brain is controlled by 2 codominant alleles with a frequency in a Caucasian population of 0.56 and 0.44. The ABAT deficiency phenotype includes psychomotor retardation, hypotonia, hyperreflexia, lethargy, refractory seizures, and EEG abnormalities. Multiple alternatively spliced transcript variants encoding the same protein isoform have been found.

 

Alternative names ABAT antibody (ab72765)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

18    (Human)

GeneCard

    

GC16P008675    (Human)

Omim

    

137150    (Human)

SwissProt

    

P80404    (Human)

Raised in 

Mouse

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Protein A purified

Storage buffer 

Preservative: None
Constituents: PBS, pH 7.2

Form 

Liquid

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this ABAT antibody is on this datasheet. But please do contact us if you would like any reassurance!


See below for ABAT antibody images, references, products related to ab72765 and other tools.

 

ABAT antibody images:

Product image  Western blot - ABAT antibody (ab72765)

Western blot - ABAT antibody (ab72765)

ABAT antibody (ab72765) at 1/500 dilution + human liver lysate at 25 µg

Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugated at 1/2500 dilution

Predicted band size : 56 kDa

 

Product image  Western blot - ABAT antibody (ab72765)

Western blot - ABAT antibody (ab72765)

All lanes : ABAT antibody (ab72765) at 1/500 dilution

Lane 1 : ABAT transfected 293T cell line lysate
Lane 2 : non transfected 293T cell line lysate

Lysates/proteins at 25 µg per lane.

Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugated at 1/2500 dilution

Predicted band size : 56 kDa

 
 

Search PubMed (MEDLINE) for references to ABAT

 

ABAT antibody - more information

Customer reviews (feedback) regarding ABAT antibody

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Price and availability of products related to ABAT antibody

 

ABAT antibody - related products:

Products for Western blot

Loading controls

Lysates

 

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ABAT antibody - other tools:

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