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Western blot - ABAT antibody (ab72765)
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Western blot - ABAT antibody (ab72765)
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Product Name
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ABAT antibody
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See all ABAT antibodies (2)...
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Product type
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Primary antibodies
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Description
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Mouse polyclonal to ABAT
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Immunogen
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Full length human ABAT protein (NP_000654.2)
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: 1/500 - 1/1000. Predicted molecular weight: 56 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Human liver lysate; lysate from ABAT transfected 293T cells.
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Cellular localization
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Mitochondrial matrix
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Research areas
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Cancer >> Cancer Metabolism >> Response to hypoxia
Signal Transduction >> Metabolism >> Mitochondrial
Neuroscience >> Neurotransmitter >> Amino Acids >> GABA
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Relevance
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4 aminobutyrate aminotransferase (ABAT) is responsible for catabolism of gamma aminobutyric acid (GABA), an important, mostly inhibitory neurotransmitter in the central nervous system, into succinic semialdehyde. The active enzyme is a homodimer of 50 kD subunits complexed to pyridoxal-5-phosphate. The protein sequence is over 95% similar to the pig protein. GABA is estimated to be present in nearly one third of human synapses. ABAT in liver and brain is controlled by 2 codominant alleles with a frequency in a Caucasian population of 0.56 and 0.44. The ABAT deficiency phenotype includes psychomotor retardation, hypotonia, hyperreflexia, lethargy, refractory seizures, and EEG abnormalities. Multiple alternatively spliced transcript variants encoding the same protein isoform have been found.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Protein A purified
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Storage buffer
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Preservative: None
Constituents: PBS, pH 7.2
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Form
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Liquid
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this ABAT antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for ABAT antibody images, references, products related to ab72765 and other tools.
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ABAT antibody images:
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 Western blot - ABAT antibody (ab72765)
ABAT antibody (ab72765) at 1/500 dilution + human liver lysate at 25 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugated at 1/2500 dilution
Predicted band size : 56 kDa
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Western blot - ABAT antibody (ab72765)
All lanes : ABAT antibody (ab72765) at 1/500 dilution
Lane 1 : ABAT transfected 293T cell line lysate
Lane 2 : non transfected 293T cell line lysate
Lysates/proteins at 25 µg per lane.
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugated at 1/2500 dilution
Predicted band size : 56 kDa
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Search PubMed (MEDLINE) for references to ABAT
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ABAT antibody - more information
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Customer reviews (feedback) regarding ABAT antibody |
Customer FAQs regarding ABAT antibody |
Protocols for ABAT antibody |
Price and availability of products related to ABAT antibody |
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ABAT antibody - related products:
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ABAT antibody - other tools:
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Contact Abcam with a Technical Enquiry about ABAT antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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