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Products:Neuroscience >> Neurotransmitter >> Amino Acids >> GABA
Overview
Product name
Anti-ABAT antibody
See all ABAT products (4) ...
Description
Rabbit polyclonal to ABAT
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Cow, Dog, Pig, Zebrafish
Immunogen
Synthetic peptide corresponding to a region within internal sequence amino acids 288-337 (AGIIVEPIQS EGGDNHASDD FFRKLRDIAR KHGCAFLVDE VQTGGGCTGK) of Human ABAT (NP_065737).
AGIIVEPIQS EGGDNHASDD FFRKLRDIAR KHGCAFLVDE VQTGGGCTGK
Positive control
Placenta lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Cancer Metabolism >> Response to hypoxia
Signal Transduction >> Metabolism >> Mitochondrial
Neuroscience >> Neurotransmitter >> Amino Acids >> GABA
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Western blot - ABAT antibody (ab81432)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab81432 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
ELISA
Application notes
ELISA titre using peptide based assay 1/12500.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Catalyzes the conversion of gamma-aminobutyrate and L-beta-aminoisobutyrate to succinate semialdehyde and methylmalonate semialdehyde, respectively. Can also convert delta-aminovalerate and beta-alanine.
Tissue specificity
Liver > pancreas > brain > kidney > heart > placenta.
Involvement in disease
Defects in ABAT are a cause of gamma-aminobutyrate transaminase deficiency (GABA-AT deficiency) [MIM:613163]. The phenotype of this deficiency includes psychomotor retardation, hypotonia, hyperreflexia, lethargy, refractory seizures, and EEG abnormalities.
Sequence similarities
Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family.
Cellular localization
Mitochondrion matrix.
Target information above from: UniProt accessionP80404
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- mitochondrial antibody
- (S) 3 amino 2 methylpropionate transaminase antibody
- (S)-3-amino-2-methylpropionate transaminase antibody
see all
Database links
- Entrez Gene: 18 Human
- Entrez Gene: 268860 Mouse
- Entrez Gene: 81632 Rat
- Omim: 137150 Human
- SwissProt: P80404 Human
- SwissProt: P61922 Mouse
- SwissProt: P50554 Rat
- Unigene: 336768 Human
see all
Anti-ABAT antibody images:
Western blot - ABAT antibody (ab81432)
Anti-ABAT antibody (ab81432) at 1 µg/ml (in 5% skim milk / PBS buffer) + Placenta lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 56 kDa
Observed band size : 56 kDa
Gel concentration 10-20%
References for Anti-ABAT antibody (ab81432)
ab81432 has not yet been referenced specifically in any publications.
Publishing research using ab81432? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"