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Western blot - ABAT antibody (ab81432)
(enlarge)
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Product Name
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ABAT antibody
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See all ABAT antibodies (2)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to ABAT
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Immunogen
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Synthetic peptide corresponding to a region within internal sequence amino acids 288-337 (AGIIVEPIQS EGGDNHASDD FFRKLRDIAR KHGCAFLVDE VQTGGGCTGK) of Human ABAT (NP_065737).
BLAST 'AGIIVEPIQS EGGDNHASDD FFRKLRDIAR KHGCAFLVDE VQTGGGCTGK ' with  or 
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Reacts with
(species key)
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Hu, Ms, Rat
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Tested applications
(see key)
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ELISA, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA titre using peptide based assay 1/12500.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Placenta lysate.
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Cellular localization
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Mitochondrial matrix
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Research areas
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Cancer >> Cancer Metabolism >> Response to hypoxia
Signal Transduction >> Metabolism >> Mitochondrial
Neuroscience >> Neurotransmitter >> Amino Acids >> GABA
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Relevance
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4 aminobutyrate aminotransferase (ABAT) is responsible for catabolism of gamma aminobutyric acid (GABA), an important, mostly inhibitory neurotransmitter in the central nervous system, into succinic semialdehyde. The active enzyme is a homodimer of 50 kD subunits complexed to pyridoxal-5-phosphate. The protein sequence is over 95% similar to the pig protein. GABA is estimated to be present in nearly one third of human synapses. ABAT in liver and brain is controlled by 2 codominant alleles with a frequency in a Caucasian population of 0.56 and 0.44. The ABAT deficiency phenotype includes psychomotor retardation, hypotonia, hyperreflexia, lethargy, refractory seizures, and EEG abnormalities. Multiple alternatively spliced transcript variants encoding the same protein isoform have been found.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: None
Constituents: 2% Sucrose, PBS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this ABAT antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for ABAT antibody images, references, products related to ab81432 and other tools.
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ABAT antibody images:
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 Western blot - ABAT antibody (ab81432)
ABAT antibody (ab81432) at 1 µg/ml (in 5% skim milk / PBS buffer) + Placenta lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 56 kDa
Observed band size : 56 kDa
Gel concentration 10-20%
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Search PubMed (MEDLINE) for references to ABAT
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ABAT antibody - more information
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Customer reviews (feedback) regarding ABAT antibody |
Customer FAQs regarding ABAT antibody |
Protocols for ABAT antibody |
Price and availability of products related to ABAT antibody |
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ABAT antibody - related products:
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ABAT antibody - other tools:
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Contact Abcam with a Technical Enquiry about ABAT antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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