ABCA1 antibody [AB.H10] (ab18180)

Product Name 

ABCA1 antibody [AB.H10]

Product type 

Primary antibodies

Description 

Mouse monoclonal [AB.H10] to ABCA1

Immunogen 

Recombinant fragment, corresponding to amino acids 1800-2260 of Human ABCA1.

Reacts with 
(species key)

Hu, Ms, Rat, Chk, Cow

Tested applications 
(see key)

ELISA, ICC/IF, IHC-P, IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
ELISA: Use at an assay dependent dilution.
ICC/IF: Use at an assay dependent concentration.
IHC-P: 1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol (in sodium citrate buffer (pH 6), steamed for 30 minutes) (see recommended protocol).Amplification with biotin-streptavidin may be required.
IP: Use at an assay dependent dilution.
WB: 1/1000. Predicted molecular weight: 254 kDa.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

testis, liver, and brain tissue
(negative control: muscle tissue)

Cellular localization 

Cell Membrane

Research areas 

Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of lipids and lipoproteins
Cardiovascular >> Atherosclerosis >> Lipoprotein metabolism
Signal Transduction >> Metabolism >> Lipid metabolism
Cardiovascular >> Lipids / Lipoproteins >> Lipid Metabolism >> Cholesterol Metabolism
Neuroscience >> Cell Type Marker >> Glia marker >> Astrocyte marker

Relevance 

This membrane-associated protein is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intracellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the ABC1 subfamily. Members of the ABC1 subfamily comprise the only major ABC subfamily found exclusively in multicellular eukaryotes. With cholesterol as its substrate, this protein functions as a cholesteral efflux pump in the cellular lipid removal pathway. Mutations in this gene have been associated with Tangier's disease and familial high-density lipoprotein deficiency.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

AB.H10

Isotype 

IgG1

Purity 

IgG fraction

Storage buffer 

Preservative: 0.05% Sodium Azide
Constituents: PBS, pH 7.4

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

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References for ABCA1 antibody [AB.H10] (ab18180)

  This product has been used in: (two most recent references)

Lagor WR et al. Overexpression of apolipoprotein F reduces HDL cholesterol levels in vivo. Arterioscler Thromb Vasc Biol 29:40-6 (2009). WB; Mouse. PubMed: 19008531

Lee TS et al. Resistin increases lipid accumulation by affecting class A scavenger receptor, CD36 and ATP-binding cassette transporter-A1 in macrophages. Life Sci 84:97-104 (2009). WB; Rat. PubMed: 19041881

See all 12 publication references for this product.


If you publish research using ab18180 please let us know so that we can cite the reference on this datasheet.

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