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Anti-ABCA1 antibody [HJ1] (ab66217)

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Overview

Product name

Anti-ABCA1 antibody [HJ1]
See all ABCA1 products (11) ...

Description

Mouse monoclonal [HJ1] to ABCA1

Tested applications

WB, Flow Cyt, IHC-Pmore details

Cross reactivity

Reacts with

Mouse, Rat, Human

Immunogen

50 kDa N-terminal extracellular loop of ABCA1

Positive control

Liver; brain.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.1% Sodium Azide
Constituents: PBS, Protease inhibitors

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Clonality

Monoclonal

Clone number

HJ1

Isotype

IgG2b

Research areas

Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of lipids and lipoproteins
Cardiovascular >> Atherosclerosis >> Lipoprotein metabolism
Signal Transduction >> Metabolism >> Lipid metabolism
Cardiovascular >> Lipids / Lipoproteins >> Lipid Metabolism >> Cholesterol Metabolism
Neuroscience >> Cell Type Marker >> Glia marker >> Astrocyte marker

  • Western blot - ABCA1 antibody [HJ1] (ab66217)Western blot - ABCA1 antibody [HJ1] (ab66217) image (enlarge)

  • Western blot - ABCA1 antibody [HJ1] (ab66217)Western blot - ABCA1 antibody [HJ1] (ab66217) image (enlarge)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ABCA1 antibody [HJ1] (ab66217)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ABCA1 antibody [HJ1] (ab66217) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab66217 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Function

cAMP-dependent and sulfonylurea-sensitive anion transporter. Key gatekeeper influencing intracellular cholesterol transport.

Tissue specificity

Widely expressed, but most abundant in macrophages.

Involvement in disease

Defects in ABCA1 are a cause of high density lipoprotein deficiency type 1 (HDLD1) [MIM:205400]; also known as analphalipoproteinemia or Tangier disease (TGD). HDLD1 is a recessive disorder characterized by absence of high density lipoprotein (HDL) cholesterol from plasma, accumulation of cholesteryl esters, premature coronary artery disease (CAD), hepatosplenomegaly, recurrent peripheral neuropathy and progressive muscle wasting and weakness.
Defects in ABCA1 are a cause of high density lipoprotein deficiency type 2 (HDLD2) [MIM:604091]; also known as familial hypoalphalipoproteinemia (FHA). HDLD2 is inherited as autosomal dominant trait. It is characterized by moderately low HDL cholesterol, predilection toward premature coronary artery disease (CAD) and a reduction in cellular cholesterol efflux.

Sequence similarities

Belongs to the ABC transporter superfamily. ABCA family.
Contains 2 ABC transporter domains.

Domain

Multifunctional polypeptide with two homologous halves, each containing an hydrophobic membrane-anchoring domain and an ATP binding cassette (ABC) domain.

Post-translational
modifications

Phosphorylation on Ser-2054 regulates phospholipid efflux.
Palmitoylation by DHHC8 is essential for membrane localization.

Cellular localization

Membrane.

Target information above from: UniProt accessionO95477 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • ABC 1 antibody
  • ABC Transporter 1 antibody
  • ABC-1 antibody
  • ABC1 antibody
  • ABCA 1 antibody
  • ABCA1 antibody
  • ABCA1_HUMAN antibody
  • ATP binding Cassette 1 antibody
  • ATP binding cassette sub family A ABC1 member 1 antibody
  • ATP binding cassette sub family A member 1 antibody
  • ATP binding cassette sub-family A member 1 antibody
  • ATP binding Cassette Transporter 1 antibody
  • ATP-binding cassette 1 antibody
  • ATP-binding cassette sub-family A member 1 antibody
  • ATP-binding cassette transporter 1 antibody
  • CERP antibody
  • Cholesterol efflux regulatory protein antibody
  • FLJ14958 antibody
  • HDLDT1 antibody
  • Membrane bound antibody
  • MGC164864 antibody
  • MGC165011 antibody
  • TD antibody
  • TGD antibody
see all

Database links

see all

Anti-ABCA1 antibody [HJ1] images:

  Western blot - ABCA1 antibody [HJ1] (ab66217)

Western blot - ABCA1 antibody [HJ1] (ab66217)

All lanes : Anti-ABCA1 antibody [HJ1] (ab66217) at 1/2000 dilution

Lane 1 : Human liver lysate
Lane 2 : Human brain lysate
Lane 3 : Rat liver lysate
Lane 4 : Rat brain lysate

Lysates/proteins at 10 µg per lane.


Predicted band size : 254 kDa

  Western blot - ABCA1 antibody [HJ1] (ab66217)

Western blot - ABCA1 antibody [HJ1] (ab66217)

All lanes : Anti-ABCA1 antibody [HJ1] (ab66217) at 1/2000 dilution

Lane 1 : wild type mouse bain lysate
Lane 2 : ABCA1 overexpressing mouse brain lysate
Lane 3 : ABCA1 (-/-) mouse liver lysate
Lane 4 : wild type mouse liver lysate

Lysates/proteins at 10 µg per lane.


Predicted band size : 254 kDa

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ABCA1 antibody [HJ1] (ab66217)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ABCA1 antibody [HJ1] (ab66217)

ab66217 (4µg/ml) staining ABCA1 in human liver using an automated system (DAKO Autostainer Plus). Using this protocol there is moderate cell membrane staining throughout the liver parenchyma.

Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

  Flow Cytometry - ABCA1 antibody [HJ1] (ab66217)

Flow Cytometry - ABCA1 antibody [HJ1] (ab66217)

Overlay histogram showing HepG2 cells stained with ab66217 (red line). The cells were fixed with methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab66217, 2µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a decreased signal in HepG2 cells fixed with 4% paraformaldehyde (10 min) used under the same conditions.

Please note that Abcam does not have data for use of this antibody on non-fixed cells. We welcome any customer feedback.

References for Anti-ABCA1 antibody [HJ1] (ab66217)

This product has been referenced in:

  • Fryirs MAet al. Effects of high-density lipoproteins on pancreatic beta-cell insulin secretion. Arterioscler Thromb Vasc Biol 30:1642-8 (2010). WB; Rat.Read more (PubMed: 20466975) »

See 1 publication for this product

Publishing research using ab66217? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"