If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab7360? Please let us know so that we can cite the reference in this datasheet
ab7360 has been referenced in 23 publications.
Publishing research using ab7360? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Predicted band size : 220 kDa
The experiment was performed by treating RAW macrophages with 9-cis-retinoic acid and 22R-hydroxycholesterol, known inducers of ABCA1expression in macrophages. Then total cell post-nuclear lysate (40ug protein) was separated by SDS-PAGE and detected using a 1:1000 dilution of ab7360 affinity purified Lot G incubated for 1 hour at room temperature (Lane A). Although there are lower molecular weight bands on the blot, the ABCA1 signal is excellent and gives the expected 3 bands. It is not known why ABCA1 runs as three bands, but it has been found to do so by many researchers. It is probably due to protein modifications such as glycosylation. The antibody was also tested against ABCA1 transiently expressed in 293 cells as an independent test with excellent results.
Predicted band size : 220 kDa
Detection of ABCA1 in mouse peritoneal macrophages using ab7360. ECL exposure, 1 min.
Lane 4: T09 uninduced lysate
Lane 5: T09 induced lysate
ICC/IF image of ab7360 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7360, 1µg/ml) overnight at +4ºC. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
All lanes : Anti-ABCA1 antibody (ab7360)
Lane 1 : RAW 264.7 whole cell lysate treated with T0901317
Lane 2 : RAW 264.7 whole cell lysate untreated
Lysates/proteins at 25 µg per lane.
Predicted band size : 220 kDa
ab7360, staining ABCA1 in prostate epithelium showing luminal and membrane staining by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
2
Call 01223 696 000 or contact us