Overview

Applications

Our Abpromise guarantee covers the use of ab7360 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
IP Use at an assay dependent dilution. PubMed: 14660648
ICC/IF Use a concentration of 1 µg/ml.
ELISA Use at an assay dependent concentration. PubMed: 21829447
WB 1/500 - 1/1000. Predicted molecular weight: 220 kDa. Additional non-specific bands are seen at lower molecular weights, but do not interfere with the ABC1 signal. It is important not to boil the sample before loading onto the gel. Boiling can cause aggregation in large proteins, resulting in the proteins inability to enter into the gel.
IHC-P 1/500.
IHC-Fr 1/750. PubMed: 12547833

Target

  • FunctioncAMP-dependent and sulfonylurea-sensitive anion transporter. Key gatekeeper influencing intracellular cholesterol transport.
  • Tissue specificityWidely expressed, but most abundant in macrophages.
  • Involvement in diseaseDefects in ABCA1 are a cause of high density lipoprotein deficiency type 1 (HDLD1) [MIM:205400]; also known as analphalipoproteinemia or Tangier disease (TGD). HDLD1 is a recessive disorder characterized by absence of high density lipoprotein (HDL) cholesterol from plasma, accumulation of cholesteryl esters, premature coronary artery disease (CAD), hepatosplenomegaly, recurrent peripheral neuropathy and progressive muscle wasting and weakness.
    Defects in ABCA1 are a cause of high density lipoprotein deficiency type 2 (HDLD2) [MIM:604091]; also known as familial hypoalphalipoproteinemia (FHA). HDLD2 is inherited as autosomal dominant trait. It is characterized by moderately low HDL cholesterol, predilection toward premature coronary artery disease (CAD) and a reduction in cellular cholesterol efflux.
  • Sequence similaritiesBelongs to the ABC transporter superfamily. ABCA family.
    Contains 2 ABC transporter domains.
  • DomainMultifunctional polypeptide with two homologous halves, each containing an hydrophobic membrane-anchoring domain and an ATP binding cassette (ABC) domain.
  • Post-translational
    modifications
    Phosphorylation on Ser-2054 regulates phospholipid efflux.
    Palmitoylation by DHHC8 is essential for membrane localization.
  • Cellular localizationMembrane.
  • Target information above from: UniProt accession O95477 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • ABC 1 antibody
    • ABC Transporter 1 antibody
    • ABC-1 antibody
    • ABC1 antibody
    • ABCA 1 antibody
    • ABCA1 antibody
    • ABCA1_HUMAN antibody
    • ATP binding Cassette 1 antibody
    • ATP binding cassette sub family A ABC1 member 1 antibody
    • ATP binding cassette sub family A member 1 antibody
    • ATP binding cassette sub-family A member 1 antibody
    • ATP binding Cassette Transporter 1 antibody
    • ATP-binding Cassette 1 antibody
    • ATP-binding cassette sub-family A member 1 antibody
    • ATP-binding Cassette Transporter 1 antibody
    • CERP antibody
    • Cholesterol Efflux Regulatory Protein antibody
    • FLJ14958 antibody
    • HDLDT1 antibody
    • Membrane bound antibody
    • MGC164864 antibody
    • MGC165011 antibody
    • TD antibody
    • TGD antibody
    see all

Anti-ABCA1 antibody images



  • Predicted band size : 220 kDa


    The experiment was performed by treating RAW macrophages with 9-cis-retinoic acid and 22R-hydroxycholesterol, known inducers of ABCA1expression in macrophages. Then total cell post-nuclear lysate (40ug protein) was separated by SDS-PAGE and detected using a 1:1000 dilution of ab7360 affinity purified Lot G incubated for 1 hour at room temperature (Lane A). Although there are lower molecular weight bands on the blot, the ABCA1 signal is excellent and gives the expected 3 bands. It is not known why ABCA1 runs as three bands, but it has been found to do so by many researchers. It is probably due to protein modifications such as glycosylation. The antibody was also tested against ABCA1 transiently expressed in 293 cells as an independent test with excellent results.


  • Predicted band size : 220 kDa


    Detection of ABCA1 in mouse peritoneal macrophages using ab7360. ECL exposure, 1 min.
    Lane 4: T09 uninduced lysate
    Lane 5: T09 induced lysate
  • ICC/IF image of ab7360 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7360, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-ABCA1 antibody (ab7360)

    Lane 1 : RAW 264.7 whole cell lysate treated with T0901317
    Lane 2 : RAW 264.7 whole cell lysate untreated

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 220 kDa
  • ab7360, staining ABCA1 in prostate epithelium showing luminal and membrane staining by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

References for Anti-ABCA1 antibody (ab7360)

This product has been referenced in:
  • Mogilenko DA  et al. Endogenous apolipoprotein A-I stabilizes ATP-binding cassette transporter A1 and modulates Toll-like receptor 4 signaling in human macrophages. FASEB J 26:2019-30 (2012). WB . Read more (PubMed: 22271762) »
  • Patel DC  et al. Type 2 Diabetes Is Associated with Reduced ATP-Binding Cassette Transporter A1 Gene Expression, Protein and Function. PLoS One 6:e22142 (2011). ELISA ; Human . Read more (PubMed: 21829447) »

See all 23 Publications for this product

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Application Western blot
Sample Human Cell lysate - whole cell (human astrocyte)
Loading amount 25 µg
Specification human astrocyte
Treatment treated with LXR ligand for 20 hrs
Gel Running Conditions Reduced Denaturing (4-15% gradient gel)
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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Submitted Aug 06 2009

Application Western blot
Sample Human Cell lysate - whole cell (HEK293, THP1, mouse BMDM, mouse tissue)
Loading amount 15 µg
Specification HEK293, THP1, mouse BMDM, mouse tissue
Treatment Transfected with ABCA1 expression plasmid, Treat with 1uM TO-1317 for 24hr
Gel Running Conditions Reduced Denaturing
Blocking step 5%milk+1%BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 4°C
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Submitted Jan 26 2008

I'm sorry to hear you are having a problem with ab7360. I would like to suggest the following modifications to your protocol: The recommended dilution range for this antibody is 1/500 - 1/1000. You may be using it at too high a dilution. I di...

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Application Western blot
Sample Mouse Tissue lysate - whole (macrophage cell line)
Loading amount 15 µg
Specification macrophage cell line
Treatment 0.3mM dbcAMP 24 hr
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5
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Submitted Sep 18 2006

Application Western blot
Sample Human Tissue lysate - other (placenta, arteries)
Loading amount 50 µg
Specification placenta, arteries
Treatment 50U Benzonuclease for 1 h
Blocking step Milk as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 7
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Submitted Aug 23 2006

Thank you for your reply and the details of the experiment. You do not seem to mention any blocking agent in the experiment and we have found that it is important to block the membrane in 5% milk for 1hr at RT, washing in TBST and adding the antibody d...

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