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Synthetic peptide: The immunogen is generated from within residues 1200-1300 of Human ABC 1
Our Abpromise guarantee covers the use of ab7360 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent dilution. PubMed: 14660648|
|ICC/IF||Use a concentration of 1 µg/ml.|
|ELISA||Use at an assay dependent concentration. PubMed: 21829447|
|WB||1/500 - 1/1000. Predicted molecular weight: 220 kDa. Additional non-specific bands are seen at lower molecular weights, but do not interfere with the ABC1 signal. It is important not to boil the sample before loading onto the gel. Boiling can cause aggregation in large proteins, resulting in the proteins inability to enter into the gel.|
|IHC-Fr||1/750. PubMed: 12547833|
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