Overview
- Product nameAnti-ABCA1 antibodySee all ABCA1 primary antibodies ...
- DescriptionRabbit polyclonal to ABCA1
- SpecificityThis antibody is specific for ABC1.
- Tested applicationsIP, ICC/IF, ELISA, WB, IHC-P, IHC-Fr more details
- Species reactivityReacts with: Mouse, Human, Monkey
- Immunogen
Synthetic peptide: The immunogen is generated from within residues 1200-1300 of Human ABC 1
Properties
- FormLiquid
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 0.02% Sodium Azide
Constituents: PBS -
Concentration information loading... - PurityImmunogen affinity purified
- Clonality Polyclonal
- IsotypeIgG
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Research Areas
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Cholesterol Metabolism
Applications
Our Abpromise guarantee covers the use of ab7360 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| IP | IP: Use at an assay dependent dilution. PubMed: 14660648 |
| ICC/IF | ICC/IF: Use a concentration of 1 µg/ml. |
| ELISA | ELISA: Use at an assay dependent concentration. PubMed: 21829447 |
| WB | WB: 1/500 - 1/1000. Predicted molecular weight: 220 kDa. Additional non-specific bands are seen at lower molecular weights, but do not interfere with the ABC1 signal. It is important not to boil the sample before loading onto the gel. Boiling can cause aggregation in large proteins, resulting in the proteins inability to enter into the gel. |
| IHC-P | IHC-P: 1/500. |
| IHC-Fr | IHC-Fr: 1/750. PubMed: 12547833 |
Target
- FunctioncAMP-dependent and sulfonylurea-sensitive anion transporter. Key gatekeeper influencing intracellular cholesterol transport.
- Tissue specificityWidely expressed, but most abundant in macrophages.
- Involvement in diseaseDefects in ABCA1 are a cause of high density lipoprotein deficiency type 1 (HDLD1) [MIM:205400]; also known as analphalipoproteinemia or Tangier disease (TGD). HDLD1 is a recessive disorder characterized by absence of high density lipoprotein (HDL) cholesterol from plasma, accumulation of cholesteryl esters, premature coronary artery disease (CAD), hepatosplenomegaly, recurrent peripheral neuropathy and progressive muscle wasting and weakness.
Defects in ABCA1 are a cause of high density lipoprotein deficiency type 2 (HDLD2) [MIM:604091]; also known as familial hypoalphalipoproteinemia (FHA). HDLD2 is inherited as autosomal dominant trait. It is characterized by moderately low HDL cholesterol, predilection toward premature coronary artery disease (CAD) and a reduction in cellular cholesterol efflux. - Sequence similaritiesBelongs to the ABC transporter superfamily. ABCA family.
Contains 2 ABC transporter domains. - DomainMultifunctional polypeptide with two homologous halves, each containing an hydrophobic membrane-anchoring domain and an ATP binding cassette (ABC) domain.
- Post-translational
modificationsPhosphorylation on Ser-2054 regulates phospholipid efflux.
Palmitoylation by DHHC8 is essential for membrane localization. - Cellular localizationMembrane.
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Database links
- Entrez Gene: 19 Human
- Entrez Gene: 11303 Mouse
- Omim: 600046 Human
- SwissProt: O95477 Human
- SwissProt: P41233 Mouse
- Unigene: 429294 Human
- Unigene: 277376 Mouse
Target information above from: UniProt accession
O95477
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- ABC 1 antibodyABC Transporter 1 antibodyABC-1 antibody
- ABC1 antibodyABCA 1 antibodyABCA1 antibodyABCA1_HUMAN antibodyATP binding Cassette 1 antibodyATP binding cassette sub family A ABC1 member 1 antibodyATP binding cassette sub family A member 1 antibodyATP binding cassette sub-family A member 1 antibodyATP binding Cassette Transporter 1 antibodyATP-binding Cassette 1 antibodyATP-binding cassette sub-family A member 1 antibodyATP-binding Cassette Transporter 1 antibodyCERP antibodyCholesterol Efflux Regulatory Protein antibodyFLJ14958 antibodyHDLDT1 antibodyMembrane bound antibodyMGC164864 antibodyMGC165011 antibodyTD antibodyTGD antibody
see all
Anti-ABCA1 antibody images
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Western blot - ABCA1 antibody (ab7360)
Predicted band size : 220 kDa
The experiment was performed by treating RAW macrophages with 9-cis-retinoic acid and 22R-hydroxycholesterol, known inducers of ABCA1expression in macrophages. Then total cell post-nuclear lysate (40ug protein) was separated by SDS-PAGE and detected using a 1:1000 dilution of ab7360 affinity purified Lot G incubated for 1 hour at room temperature (Lane A). Although there are lower molecular weight bands on the blot, the ABCA1 signal is excellent and gives the expected 3 bands. It is not known why ABCA1 runs as three bands, but it has been found to do so by many researchers. It is probably due to protein modifications such as glycosylation. The antibody was also tested against ABCA1 transiently expressed in 293 cells as an independent test with excellent results. -
Western blot - ABCA1 antibody (ab7360)
Predicted band size : 220 kDa
Detection of ABCA1 in mouse peritoneal macrophages using ab7360. ECL exposure, 1 min.
Lane 4: T09 uninduced lysate
Lane 5: T09 induced lysate -
Immunocytochemistry/ Immunofluorescence - ABCA1 antibody (ab7360)ICC/IF image of ab7360 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7360, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. -
Western blot - ABCA1 antibody (ab7360)All lanes : Anti-ABCA1 antibody (ab7360)
Lane 1 : RAW 264.7 whole cell lysate treated with T0901317
Lane 2 : RAW 264.7 whole cell lysate untreated
Lysates/proteins at 25 µg per lane.
Predicted band size : 220 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ABCA1 antibody (ab7360)ab7360, staining ABCA1 in prostate epithelium showing luminal and membrane staining by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
Protocols
References for Anti-ABCA1 antibody (ab7360)
This product has been referenced in:
- Mogilenko DA et al. Endogenous apolipoprotein A-I stabilizes ATP-binding cassette transporter A1 and modulates Toll-like receptor 4 signaling in human macrophages. FASEB J 26:2019-30 (2012). WB . Read more (PubMed: 22271762) »
- Patel DC et al. Type 2 Diabetes Is Associated with Reduced ATP-Binding Cassette Transporter A1 Gene Expression, Protein and Function. PLoS One 6:e22142 (2011). ELISA ; Human . Read more (PubMed: 21829447) »
