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Western blot - ABCA1 antibody (ab7360)
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Western blot - ABCA1 antibody (ab7360)
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Immunocytochemistry/ Immunofluorescence-ABCA1 antibody(ab7360)
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Product Name
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ABCA1 antibody
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See all ABCA1 antibodies (10)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to ABCA1
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Immunogen
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Synthetic peptide: The immunogen is generated from within residues 1200-1300 of Human ABC 1
BLAST 'AETSDGTLPAP' with  or 
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Reacts with
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Hu, Ms, Mk
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Specificity
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This antibody is specific for ABC1.
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Tested applications
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ICC/IF, IHC-Fr, IHC-P, IP, WB
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Abreviews
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Application notes
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Recommended dilutions
ICC/IF: Use at a concentration of 1 µg/ml.
IHC-P: 1/500. Please see PubMed ID: 12586329
IHC-Fr: 1/750. Please see PubMed ID: 12547833.
IP: Use at an assay dependent dilution. Please see PubMed IDs: 14660648 and 14754908.
WB: 1/500 - 1/1000. Predicted molecular weight: 220 kDa. Additional non-specific bands are seen at lower molecular weights, but do not interfere with the ABC1 signal.
It is important not to boil the sample before loading onto the gel. Boiling can cause aggregation in large proteins, resulting in the proteins inability to enter into the gel.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Cell Membrane
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Research areas
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Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of lipids and lipoproteins
Cardiovascular >> Atherosclerosis >> Lipoprotein metabolism
Signal Transduction >> Metabolism >> Lipid metabolism
Cardiovascular >> Lipids / Lipoproteins >> Lipid Metabolism >> Cholesterol Metabolism
Neuroscience >> Cell Type Marker >> Glia marker >> Astrocyte marker
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Relevance
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This membrane-associated protein is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intracellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the ABC1 subfamily. Members of the ABC1 subfamily comprise the only major ABC subfamily found exclusively in multicellular eukaryotes. With cholesterol as its substrate, this protein functions as a cholesteral efflux pump in the cellular lipid removal pathway. Mutations in this gene have been associated with Tangier's disease and familial high-density lipoprotein deficiency.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: PBS Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this ABCA1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for ABCA1 antibody images, references, products related to ab7360 and other tools.
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ABCA1 antibody images:
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 Western blot - ABCA1 antibody (ab7360)
Predicted band size : 220 kDa
The experiment was performed by treating RAW macrophages with 9-cis-retinoic acid and 22R-hydroxycholesterol, known inducers of ABCA1expression in macrophages. Then total cell post-nuclear lysate (40ug protein) was separated by SDS-PAGE and detected using a 1:1000 dilution of ab7360 affinity purified Lot G incubated for 1 hour at room temperature (Lane A). Although there are lower molecular weight bands on the blot, the ABCA1 signal is excellent and gives the expected 3 bands. It is not known why ABCA1 runs as three bands, but it has been found to do so by many researchers. It is probably due to protein modifications such as glycosylation. The antibody was also tested against ABCA1 transiently expressed in 293 cells as an independent test with excellent results.
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Western blot - ABCA1 antibody (ab7360)
Predicted band size : 220 kDa
Detection of ABCA1 in mouse peritoneal macrophages using ab7360. ECL exposure, 1 min.
Lane 4: T09 uninduced lysate
Lane 5: T09 induced lysate
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Immunocytochemistry/ Immunofluorescence-ABCA1 antibody(ab7360)
ICC/IF image of ab7360 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7360, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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References for ABCA1 antibody (ab7360)
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This product has been used in: (two most recent references)
Mani O et al. Differential expression and localization of lipid transporters in the bovine mammary gland during the pregnancy-lactation cycle. J Dairy Sci 92:3744-56 (2009). IHC-P. PubMed: 19620656
Kasza I et al. Model system for the analysis of cell surface expression of human ABCA1. BMC Cell Biol 10:93 (2009). WB; Human. PubMed: 20025763
See all 19 publication references for this product.
If you publish research using ab7360 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to ABCA1
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ABCA1 antibody - more information
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