ABCA2 antibody [9A2-51.3] (FITC) (ab80229)

Product Name 

ABCA2 antibody [9A2-51.3] (FITC)

Product type 

Primary antibodies

Description 

Rat monoclonal [9A2-51.3] to ABCA2 (FITC)

Immunogen 

ABCA2 transfected HeLa cells

Reacts with 
(species key)

Ms

Specificity 

Recognises murine ABCA2.

Tested applications 
(see key)

Flow Cyt

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
Flow Cyt: Neat - 1/10. Use 10µl of the working dilution to label 10⁶ cells per 100µl. Membrane permeabilisation may improve staining with this antibody.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Mouse ABCA2 transfected HeLa cells (permeabilised)

Cellular localization 

Plasma membrane

Research areas 

Cardiovascular >> Atherosclerosis >> Lipid transport
Signal Transduction >> Metabolism >> Lipid metabolism
Neuroscience >> Neurology process >> Neurogenesis
Signal Transduction >> Signaling Pathway >> Lipid Signaling >> Other
Cardiovascular >> Lipids / Lipoproteins >> Lipid Metabolism >> Cholesterol Metabolism
Immunology >> Innate Immunity >> Macrophage / Inflamm.

Relevance 

The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intracellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the ABC1 subfamily. Members of the ABC1 subfamily comprise the only major ABC subfamily found exclusively in multicellular eukaryotes. This protein is highly expressed in brain tissue and may play a role in macrophage lipid metabolism and neural development. Two transcript variants encoding different isoforms have been found for this gene (from EntrezGene).

Database links 

Raised in 

Rat

Clonality 

Monoclonal

Clone number 

9A2-51.3

Isotype 

IgG2a

Purity 

Protein G purified

Storage buffer 

Preservative: 0.09% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Purification notes 

Prepared by affinity chromatography on Protein G from tissue culture supernatant.

Form 

Liquid

Conjugation 

Fluorescein

Concentration 

0.100 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C. Store in the dark. Avoid repeated freeze / thaw cycles.

Notes 

This product should be stored undiluted.
Shelf Life :18 months from date of despatch.

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