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ab77285, at a 1/100 dilution, staining ABCA4 in formalin fixed, paraffin embedded adult mouse retina tissue by Immunohistochemisty.
All lanes : Anti-ABCA4 antibody [3F4] (ab77285) at 1/500 dilution
Lane 1 : WERI (Human Retinoblastoma) Whole Cell Lysate
Lane 2 : Y79 (Human retinoblastoma cell line) Whole Cell Lysate
Lane 3 : Rat Retina Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed (ab97040) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 257 kDa
Observed band size : 257 kDa
Additional bands at : 142 kDa,51 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 20 minutes
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