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ab87350
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Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab72955? Please let us know so that we can cite the reference in this datasheet
ab72955 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - ABCA4 antibody (ab72955)
All lanes : Anti-ABCA4 antibody (ab72955) at 1 µg/ml
Lane 1 : Rat Retina Tissue Lysate
Lane 2 : Mouse Retina Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 256 kDa
Observed band size : 230 kDa (why is the actual band size different from the predicted?)
Additional bands at : 41 kDa. We are unsure as to the identity of these extra bands.
ABCA4 contains a number of potential glycosylation sites (SwissProt) which may explain the migration pattern observed. The 230 kDa band observed is comparable to the molecular weight seen with other commercially available antibodies to the same target.
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