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Flow Cytometry - ABCA7 antibody [7A1-144] (ab48265)
![Flow Cytometry - ABCA7 antibody [7A1-144] (ab48265)](/ps/datasheet/images/ab48265_1.jpg)
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Western blot - ABCA7 antibody [7A1-144] (ab48265)
![Western blot - ABCA7 antibody [7A1-144] (ab48265)](/ps/datasheet/images/ab48265.jpg)
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Product Name
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ABCA7 antibody [7A1-144]
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See all ABCA7 antibodies (2)...
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Product type
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Primary antibodies
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Description
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Rat monoclonal [7A1-144] to ABCA7
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Immunogen
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Mouse ABCA7 stably-transfected HeLa cells.
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Reacts with
(species key)
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Ms
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Specificity
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This antibody is specific for ABCA7
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Epitope
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Extracellular determinants on ABCA7 of an unknown position
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Tested applications
(see key)
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Flow Cyt, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions Flow Cyt: 1/10000 - 1/60000. WB: 1/2000 - 1/8000. Detects a band of approximately 240 kDa (predicted molecular weight: 234 kDa). This product is known to aggregate. Do not boil samples. Use a reducing buffer: 2X sample buffer): 8M Urea, 2% SDS, 715 mM BME, 1% glycerol, 0.001% Bromophenol Blue, 125 mM Tris-HCl [pH 6.8].
Is unsuitable for ELISA.
PLEASE NOTE that this product was tested using recombinant protein only.
Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Cell Membrane and Cytoplasmic
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Research areas
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Signal Transduction >> Metabolism >> Lipid metabolism Signal Transduction >> Metabolism >> Plasma Membrane >> ATPases Cardiovascular >> Lipids / Lipoproteins >> Lipid Metabolism >> Cholesterol Metabolism Neuroscience >> Cell Type Marker >> Glia marker >> Astrocyte marker
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Relevance
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ATP-binding cassette (ABC)1 transporters are a superfamily of highly conserved membrane proteins that transport a wide variety of substrates including ions, amino acids, peptides, sugars, lipids, and sterols across cell membranes.
ABCA7 is a full-size ABC transporter, the physiological function of which is unclear. This is a protein that shows the highest homology known to ABCA1, an essential molecule for producing of plasma high-density lipoprotein (HDL), and in fact it mimics ABCA1 to mediate the production of HDL from cellular lipid when transfected in vitro. ABCA7 might play a relevant role in regulating lipid metabolism.
Mutations of ABC transporters have been identified as the cause of at lease 12 different genetic disorders.
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Database links
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The links below go to external sites and will open in a new browser window Entrez Gene | | 27403 (Mouse)
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Raised in
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Rat
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Clonality
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Monoclonal
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Clone number
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7A1-144
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Isotype
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IgG2a
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Light chain type
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kappa
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Purity
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Ascites
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Storage buffer
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Preservative: 0.1% Sodium Azide Constituents: Ascites Material safety datasheet (MSDS) for this product: Sodium Azide MSDS
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Form
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Liquid
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Concentration
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37.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this ABCA7 antibody [7A1-144] is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for ABCA7 antibody [7A1-144] images, references, products related to ab48265 and other tools.
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ABCA7 antibody [7A1-144] images:
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Flow Cytometry - ABCA7 antibody [7A1-144] (ab48265)
FACS analysis of 6C (red) (negative control HeLa cells) and A7(blue) (ABCA7 expressing HeLa cells) using ab48265 at a dilution of 1/3000
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Western blot - ABCA7 antibody [7A1-144] (ab48265)
All lanes : ABCA7 antibody [7A1-144] (ab48265)
Lane 1 : ab48265 (1/2000) + Hela cell lysate transfected with mouse ABCA7 Lane 2 : ab48265 (1/4000) + Hela cell lysate transfected with mouse ABCA7 Lane 3 : ab48265 (1/8000) + Hela cell lysate transfected with mouse ABCA7 Lane 4 : ab48265 (1/16000) + Hela cell lysate transfected with mouse ABCA7
developed using the ECL technique
Predicted band size : 234 kDa
Please note that this antibody has only been tested using recombinant mouse ABCA7 protein.
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Search PubMed (MEDLINE) for references to ABCA7
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ABCA7 antibody [7A1-144] - more information
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Customer reviews (feedback) regarding ABCA7 antibody [7A1-144] |
Customer FAQs regarding ABCA7 antibody [7A1-144] |
Protocols for ABCA7 antibody [7A1-144] |
Price and availability of products related to ABCA7 antibody [7A1-144] |
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ABCA7 antibody [7A1-144] - related products:
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ABCA7 antibody [7A1-144] - other tools:
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Contact Abcam with a Technical Enquiry about ABCA7 antibody [7A1-144] |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"