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Western blot - ABCA8 antibody (ab74809)
(enlarge)
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Product Name
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ABCA8 antibody
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to ABCA8
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Immunogen
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Synthetic peptide derived from an internal sequence of human ABCA8.
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Reacts with
(species key)
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Hu, Ms
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Specificity
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ab74809 detects endogenous levels of total ABCA8 protein.
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Tested applications
(see key)
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ELISA, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/40000.
WB: 1/500 - 1/1000. Predicted molecular weight: 179 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Extracts from HUVEC cells.
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Cellular localization
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Cell membrane; Multi pass membrane protein
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Research areas
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Signal Transduction >> Metabolism >> Drug metabolism
Signal Transduction >> Metabolism >> Plasma Membrane >> ATPases
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Relevance
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ABCA8 is a member of the superfamily of ATP binding cassette (ABC) transporters. ABC proteins transport various molecules across extra and intracellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the ABC1 subfamily. Members of the ABC1 subfamily comprise the only major ABC subfamily found exclusively in multicellular eukaryotes. The function of this protein has not yet been determined.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+),150mM Sodium chloride, pH 7.4 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Purification notes
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The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this ABCA8 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for ABCA8 antibody images, references, products related to ab74809 and other tools.
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ABCA8 antibody images:
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 Western blot - ABCA8 antibody (ab74809)
All lanes : ABCA8 antibody (ab74809) at 1/500 dilution
Lane 1 : extracts from HUVEC cells
Lane 2 : extracts from HUVEC cells with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size : 179 kDa
Observed band size : >170 kDa (why is the actual band size different from the predicted?)
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Search PubMed (MEDLINE) for references to ABCA8
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ABCA8 antibody - more information
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Customer reviews (feedback) regarding ABCA8 antibody |
Customer FAQs regarding ABCA8 antibody |
Protocols for ABCA8 antibody |
Price and availability of products related to ABCA8 antibody |
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ABCA8 antibody - related products:
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ABCA8 antibody - other tools:
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Contact Abcam with a Technical Enquiry about ABCA8 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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