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Anti-ABCA8 antibody (ab74809)

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Overview

Product name

Anti-ABCA8 antibody

Description

Rabbit polyclonal to ABCA8

Specificity

ab74809 detects endogenous levels of total ABCA8 protein.

Tested applications

WB, ELISAmore details

Cross reactivity

Reacts with

Human

Predicted to work with

Mouse

Immunogen

Synthetic peptide derived from an internal sequence of human ABCA8.

Positive control

Extracts from HUVEC cells.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+),150mM Sodium chloride, pH 7.4

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Purification notes

The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.

Clonality

Polyclonal

Isotype

IgG

Research areas

Signal Transduction >> Metabolism >> Drug metabolism
Signal Transduction >> Metabolism >> Plasma Membrane >> ATPases

  • Western blot - ABCA8 antibody (ab74809)Western blot - ABCA8 antibody (ab74809) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab74809 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide1 Image

    WB

     WB: 1/500 - 1/1000.Predi...Read more →

    WB: 1/500 - 1/1000.Predicted molecular weight: 179 kDa.

  • ELISA

     ELISA: 1/40000

    ELISA: 1/40000

Target

Relevance

ABCA8 is a member of the superfamily of ATP binding cassette (ABC) transporters. ABC proteins transport various molecules across extra and intracellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the ABC1 subfamily. Members of the ABC1 subfamily comprise the only major ABC subfamily found exclusively in multicellular eukaryotes. The function of this protein has not yet been determined.

Cellular localization

Cell membrane; Multi pass membrane protein

Alternative names

  • ATP binding cassette sub family A member 8 antibody
  • ABCA 8 antibody
  • ATP binding cassette, sub family A (ABC1), member 8 antibody

Database links

Anti-ABCA8 antibody images:

  Western blot - ABCA8 antibody (ab74809)

Western blot - ABCA8 antibody (ab74809)

All lanes : Anti-ABCA8 antibody (ab74809) at 1/500 dilution

Lane 1 : extracts from HUVEC cells
Lane 2 : extracts from HUVEC cells with immunising peptide at 5 µg

Lysates/proteins at 5 µg per lane.


Predicted band size : 179 kDa
Observed band size : >170 kDa (why is the actual band size different from the predicted?)

References for Anti-ABCA8 antibody (ab74809)

ab74809 has not yet been referenced specifically in any publications.

Publishing research using ab74809? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"