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Read our guarantee »Products:Signal Transduction >> Metabolism >> Plasma Membrane >> ATPases
Anti-ABCA8 antibody
Rabbit polyclonal to ABCA8
ab74809 detects endogenous levels of total ABCA8 protein.
WB, ELISAmore details
Reacts with
Human
Predicted to work with
Mouse
Synthetic peptide derived from an internal sequence of human ABCA8.
Extracts from HUVEC cells.
Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+),150mM Sodium chloride, pH 7.4
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Immunogen affinity purified
The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
Polyclonal
IgG
Signal Transduction >> Metabolism >> Drug metabolism
Signal Transduction >> Metabolism >> Plasma Membrane >> ATPases
Western blot - ABCA8 antibody (ab74809)
(enlarge)
Our Abpromise guarantee covers the use of ab74809 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000.Predicted molecular weight: 179 kDa.
ELISA: 1/40000
ABCA8 is a member of the superfamily of ATP binding cassette (ABC) transporters. ABC proteins transport various molecules across extra and intracellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the ABC1 subfamily. Members of the ABC1 subfamily comprise the only major ABC subfamily found exclusively in multicellular eukaryotes. The function of this protein has not yet been determined.
Cell membrane; Multi pass membrane protein
Western blot - ABCA8 antibody (ab74809)

All lanes : Anti-ABCA8 antibody (ab74809) at 1/500 dilution
Lane 1 : extracts from HUVEC cells
Lane 2 : extracts from HUVEC cells with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size : 179 kDa
Observed band size : >170 kDa (why is the actual band size different from the predicted?)
ab74809 has not yet been referenced specifically in any publications.
Publishing research using ab74809? Please let us know so that we can cite the reference in this datasheet
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All lanes : Anti-ABCA8 antibody (ab74809) at 1/500 dilution
Lane 1 : extracts from HUVEC cells
Lane 2 : extracts from HUVEC cells with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size : 179 kDa
Observed band size : >170 kDa (why is the actual band size different from the predicted?)
0
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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