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Anti-ABCA9 antibody (ab81902) at 0.1 µg/ml + Human Cerebellum lysate in RIPA buffer at 35 µg
developed using the ECL technique
Predicted band size : 184 kDa
Observed band size : 170 kDa (why is the actual band size different from the predicted?)
Primary incubation was 1 hour.
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