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Western blot - ABCA9 antibody (ab81902)
(enlarge)
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Product Name
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ABCA9 antibody
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Product type
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Primary antibodies
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Description
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Goat polyclonal to ABCA9
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Immunogen
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Synthetic peptide: C-QRVVQELEMENIQD, corresponding to internal sequence amino acids 599-612 of Human ABCA9 (NP_525022.2).
BLAST 'C-QRVVQELEMENIQD' with  or 
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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ELISA, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
Peptide ELISA: Antibody detection limit dilution 1/128000.
WB: Use at a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 170 kDa (predicted molecular weight: 184 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Human Brain (Cerebellum) lysate.
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Cellular localization
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Membrane; Multi-pass membrane protein
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Research areas
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Signal Transduction >> Metabolism >> Lipid metabolism
Cell Biology >> Cell Cycle >> Cell differentiation
Signal Transduction >> Metabolism >> Plasma Membrane >> ATPases
Immunology >> Innate Immunity >> Macrophage / Inflamm.
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Relevance
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ABCA9 is a member of the superfamily of ATP-binding cassette (ABC) transporters and contains two transmembrane domains and two nucleotide binding folds. ABC proteins transport various molecules across extra- and intracellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, and White). ABCA9 is a member of the ABC1 subfamily and is clustered with four other ABC1 family members on chromosome 17q24. Transcriptional expression of the ABCA9 gene is induced during monocyte differentiation into macrophages and is suppressed by cholesterol import. ABCA9 May play a role in monocyte differentiation and lipid homeostasis.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Goat
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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ab81902 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this ABCA9 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for ABCA9 antibody images, references, products related to ab81902 and other tools.
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ABCA9 antibody images:
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 Western blot - ABCA9 antibody (ab81902)
ABCA9 antibody (ab81902) at 0.1 µg/ml + Human Cerebellum lysate in RIPA buffer at 35 µg
developed using the ECL technique
Predicted band size : 184 kDa
Observed band size : 170 kDa (why is the actual band size different from the predicted?)
Primary incubation was 1 hour.
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Search PubMed (MEDLINE) for references to ABCA9
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ABCA9 antibody - more information
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Customer reviews (feedback) regarding ABCA9 antibody |
Customer FAQs regarding ABCA9 antibody |
Protocols for ABCA9 antibody |
Price and availability of products related to ABCA9 antibody |
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ABCA9 antibody - related products:
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ABCA9 antibody - other tools:
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Contact Abcam with a Technical Enquiry about ABCA9 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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