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Products:Immunology >> Innate Immunity >> Macrophage / Inflamm.
Overview
Product name
Anti-ABCA9 antibody
Description
Goat polyclonal to ABCA9
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide: C-QRVVQELEMENIQD, corresponding to internal sequence amino acids 599-612 of Human ABCA9 (NP_525022.2).
C-QRVVQELE MENIQD
Positive control
Human Brain (Cerebellum) lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
ab81902 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Metabolism >> Lipid metabolism
Cell Biology >> Cell Cycle >> Cell differentiation
Signal Transduction >> Metabolism >> Plasma Membrane >> ATPases
Immunology >> Innate Immunity >> Macrophage / Inflamm.
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Western blot - ABCA9 antibody (ab81902)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab81902 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
ELISA
Application notes
Peptide ELISA: Antibody detection limit dilution 1/128000.
WB: Use at a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 170 kDa (predicted molecular weight: 184 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
ABCA9 is a member of the superfamily of ATP-binding cassette (ABC) transporters and contains two transmembrane domains and two nucleotide binding folds. ABC proteins transport various molecules across extra- and intracellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, and White). ABCA9 is a member of the ABC1 subfamily and is clustered with four other ABC1 family members on chromosome 17q24. Transcriptional expression of the ABCA9 gene is induced during monocyte differentiation into macrophages and is suppressed by cholesterol import. ABCA9 May play a role in monocyte differentiation and lipid homeostasis.
Cellular localization
Membrane; Multi-pass membrane protein
Alternative names
- ATP binding cassette A9 antibody
- ATP binding cassette sub family A (ABC1) member 9 antibody
- ATP binding cassette sub family A member 9 antibody
see all
Database links
- Entrez Gene: 10350 Human
- Omim: 612507 Human
- SwissProt: Q8IUA7 Human
- Unigene: 131686 Human
Anti-ABCA9 antibody images:
Western blot - ABCA9 antibody (ab81902)
Anti-ABCA9 antibody (ab81902) at 0.1 µg/ml + Human Cerebellum lysate in RIPA buffer at 35 µg
developed using the ECL technique
Predicted band size : 184 kDa
Observed band size : 170 kDa (why is the actual band size different from the predicted?)
Primary incubation was 1 hour.
References for Anti-ABCA9 antibody (ab81902)
ab81902 has not yet been referenced specifically in any publications.
Publishing research using ab81902? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"