Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Signal Transduction >> Metabolism >> Mitochondrial
Overview
Product name
Anti-ABCB10 antibody
See all ABCB10 products (2) ...
Description
Rabbit polyclonal to ABCB10
Specificity
ab74815 detects endogenous levels of total ABCB10 protein.
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse
Immunogen
Synthetic peptide derived from an internal sequence of human ABCB10.
Positive control
Extracts from COLO cells.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
Clonality
Polyclonal
Isotype
IgG
Research areas
-
Western blot - ABCB10 antibody (ab74815)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab74815 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000.Predicted molecular weight: 79 kDa.
ELISA
ELISA: 1/40000ELISA: 1/40000
Target
Function
May mediate critical mitochondrial transport functions related to heme biosynthesis.
Tissue specificity
Ubiquitous. Highly expressed in bone marrow, expressed at intermediate to high levels in skeletal muscle, small intestine, thyroid, heart, brain, placenta, liver, pancreas, prostate, testis, ovary, leukocyte, stomach, spinal cord, lymph node, trachea and adrenal gland, and low levels are found in lung, kidney, spleen, thymus and colon.
Sequence similarities
Belongs to the ABC transporter superfamily. ABCB family. Mitochondrial peptide exporter (TC 3.A.1.212) subfamily.
Contains 1 ABC transmembrane type-1 domain.
Contains 1 ABC transporter domain.
Cellular localization
Mitochondrion inner membrane.
Target information above from: UniProt accessionQ9NRK6
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- mitochondrial antibody
- ABC transporter 10 protein antibody
- ABCB10 antibody
see all
Database links
- Entrez Gene: 23456 Human
- Entrez Gene: 56199 Mouse
- Omim: 605454 Human
- SwissProt: Q9NRK6 Human
- SwissProt: Q9JI39 Mouse
- Unigene: 17614 Human
- Unigene: 274243 Mouse
Anti-ABCB10 antibody images:
Western blot - ABCB10 antibody (ab74815)
All lanes : Anti-ABCB10 antibody (ab74815) at 1/500 dilution
Lane 1 : extracts from COLO cells
Lane 2 : extracts from COLO cells with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size : 79 kDa
Observed band size : 79 kDa
Additional bands at : 116 kDa,60 kDa. We are unsure as to the identity of these extra bands.
References for Anti-ABCB10 antibody (ab74815)
ab74815 has not yet been referenced specifically in any publications.
Publishing research using ab74815? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"