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Western blot - ABCB10 antibody (ab74815)
Western blot - ABCB10 antibody (ab74815)
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Product Name 

ABCB10 antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to ABCB10

Immunogen 

Synthetic peptide derived from an internal sequence of human ABCB10.

Reacts with 
(species key)

Hu, Ms

Specificity 

ab74815 detects endogenous levels of total ABCB10 protein.

Tested applications 
(see key)

ELISA, WB


Abreviews 

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(see key)


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Application notes 
(see key)

Recommended dilutions
ELISA: 1/40000.
WB: 1/500 - 1/1000. Predicted molecular weight: 79 kDa.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Extracts from COLO cells.

Cellular localization 

Mitochondrion inner membrane; Multi pass membrane protein.

Research areas 

Signal Transduction >> Metabolism >> Mitochondrial

Relevance 

ABCB10 is a member of the superfamily of ATP binding cassette (ABC) transporters. It may be a mitochondrial inner membrane erythroid transporter involved in heme biosynthesis. ABC proteins transport various molecules across extra and intra cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20). This protein is a member of the MDR/TAP subfamily which are involved in multidrug resistance. The exact function of this protein is unknown.

 

Alternative names ABCB10 antibody (ab74815)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

23456    (Human)
56199    (Mouse)

GeneCard

    

GC01M227718    (Human)

Omim

    

605454    (Human)

SwissProt

    

Q9NRK6    (Human)
Q9JI39    (Mouse)

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Purification notes 

The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this ABCB10 antibody is on this datasheet. But please do contact us if you would like any reassurance!


See below for ABCB10 antibody images, references, products related to ab74815 and other tools.

 

ABCB10 antibody images:

Product image  Western blot - ABCB10 antibody (ab74815)

Western blot - ABCB10 antibody (ab74815)

All lanes : ABCB10 antibody (ab74815) at 1/500 dilution

Lane 1 : extracts from COLO cells
Lane 2 : extracts from COLO cells with immunising peptide at 5 µg

Lysates/proteins at 5 µg per lane.


Predicted band size : 79 kDa
Observed band size : 79 kDa
Additional bands at : 116 kDa,60 kDa. We are unsure as to the identity of these extra bands.

 
 

Search PubMed (MEDLINE) for references to ABCB10

 

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