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Western blot - ABCB10 antibody (ab74815)

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Product Name
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ABCB10 antibody
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to ABCB10
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Immunogen
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Synthetic peptide derived from an internal sequence of human ABCB10.
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Reacts with
(species key)
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Hu, Ms
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Specificity
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ab74815 detects endogenous levels of total ABCB10 protein.
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Tested applications
(see key)
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ELISA, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions ELISA: 1/40000. WB: 1/500 - 1/1000. Predicted molecular weight: 79 kDa.
Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Extracts from COLO cells.
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Cellular localization
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Mitochondrion inner membrane; Multi pass membrane protein.
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Research areas
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Signal Transduction >> Metabolism >> Mitochondrial
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Relevance
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ABCB10 is a member of the superfamily of ATP binding cassette (ABC) transporters. It may be a mitochondrial inner membrane erythroid transporter involved in heme biosynthesis. ABC proteins transport various molecules across extra and intra cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20). This protein is a member of the MDR/TAP subfamily which are involved in multidrug resistance. The exact function of this protein is unknown.
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Database links
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The links below go to external sites and will open in a new browser window
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4 Material safety datasheets (MSDS) for this product: Glycerol MSDS Sodium Azide MSDS
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Purification notes
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The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this ABCB10 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for ABCB10 antibody images, references, products related to ab74815 and other tools.
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ABCB10 antibody images:
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Western blot - ABCB10 antibody (ab74815)
All lanes : ABCB10 antibody (ab74815) at 1/500 dilution
Lane 1 : extracts from COLO cells Lane 2 : extracts from COLO cells with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size : 79 kDa Observed band size : 79 kDa Additional bands at : 116 kDa,60 kDa. We are unsure as to the identity of these extra bands.
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Search PubMed (MEDLINE) for references to ABCB10
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ABCB10 antibody - more information
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Customer reviews (feedback) regarding ABCB10 antibody |
Customer FAQs regarding ABCB10 antibody |
Protocols for ABCB10 antibody |
Price and availability of products related to ABCB10 antibody |
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ABCB10 antibody - related products:
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ABCB10 antibody - other tools:
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Contact Abcam with a Technical Enquiry about ABCB10 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"