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Please help me with this antibody- ABCB111 (ab71793), its not working. I am using LSAB with 1:100 dilution , HEIR @ high pH. Thanks |
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ANSWER: |
Thank you for contacting us. I am sorry to hear you are experiencing difficulties with one of our products and that you do not observe a staining in IHC. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly. I am attaching our questionnaire so that we can gather further information regarding the samples tested and the protocol used. Once we receive the completed questionnaire, we will look at the protocol and see if there are any suggestions we can make that may improve the results. This information will also allow us to investigate this case internally and initiate additional testing where necessary. If the product was purchased in the last six months and is being used according to our Abpromise, we would be happy to replace or refund the antibody. I look forward to receiving your reply. |
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Hi, I would like to know if the epitope is in the exposed extracellular or intracellular domain of the C-terminus. I am interested to use this antibody on membrane vesicle preps to determine orientation of the membrane sidedness of the vesicle preps. Thanks |
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ANSWER: |
Thank you for your enquiry. I have contacted the originator of the product for more information, unfortunately the exact immunogen sequence information is proprietary. However, they did released information that the sequence is a 15 amino acid protein taken between amino acids 1025 and 1075. I hope this information will still be helpful to you. If there is anything else that I can help you with, please do not hesitate to contact me. |
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