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Western blot - ABCB11 antibody - Carboxyterminal end (ab71793)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ABCB11 antibody - Carboxyterminal end (ab71793)

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Product Name
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ABCB11 antibody - Carboxyterminal end
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to ABCB11 - Carboxyterminal end
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Immunogen
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A KLH conjugated synthetic peptide selected from the C-terminal region of human ABCB11.
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Reacts with
(species key)
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Hu, Ms
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Tested applications
(see key)
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ELISA, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions ELISA: 1/1000. IHC-P: 1/50 - 1/100. WB: 1/100 - 1/500. Detects a band of approximately 146 kDa plus a range of other bands. (predicted molecular weight: 146 kDa).
Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Human hepatocarcinoma tissue and mouse liver tissue lysate.
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Cellular localization
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Multi pass membrane protein
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Research areas
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Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of lipids and lipoproteins Signal Transduction >> Metabolism >> Lipid metabolism Signal Transduction >> Metabolism >> Plasma Membrane >> Channels
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Relevance
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Bile formation is an important function of the liver. It is mediated by hepatocytes which generate bile flow within the bile canaliculi by continuous vectorial secretion of bile salts and other solutes across their canalicular (apical) membrane. Bile secretion is mediated by several ATP-binding cassette (ABC) transporters located in the canalicular membrane of hepatocytes. Among these ABC transporters, ABCB11 (bile salt export pump or BSEP) represents the primary, if not sole transport system for the canalicular excretion of bile salts. Bile secretory failure results in cholestasis and progressive familial intrahepatic cholestasis (PFIC) in infancy represents a group of inherited cholestatic diseases that are classified into three subtypes. One of these subtypes, PFIC II, is associated with mutations in the ABCB11 gene. PFIC patients with mutations in the ABCB11 gene have normal serum gamma-glutamyltransferase activity, low concentrations of bile salts in bile, and an absence of bile duct proliferation. Additionally, human obesity is associated with altered cholesterol homeostasis including increased production and turnover, as well as secretion of excess cholesterol from the liver into bile. ABCB11 is a multifunctional polypeptide with two homologous halves, each containing a hydrophobic membrane ancoring domain and an ATP binding cassette (ABC) domain. The membrane anchoring domain is composed of six helixes buried in the lipid bilayer of the plasma membrane and the ATP binding ABCs are exposed to the cytosol. The membrane anchoring domain helixes are thought to form channels spanning the plasma membrane.
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Database links
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The links below go to external sites and will open in a new browser window
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Protein G purified
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Storage buffer
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Preservative: 0.09% Sodium Azide Constituents: PBS Material safety datasheet (MSDS) for this product: Sodium Azide MSDS
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Purification notes
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This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Form
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Liquid
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Concentration
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0.250 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this ABCB11 antibody - Carboxyterminal end is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for ABCB11 antibody - Carboxyterminal end images, references, products related to ab71793 and other tools.
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ABCB11 antibody - Carboxyterminal end images:
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Western blot - ABCB11 antibody - Carboxyterminal end (ab71793)
ABCB11 antibody - Carboxyterminal end (ab71793) at 1/100 dilution + mouse liver tissue lysate at 12.5 µg
Predicted band size : 146 kDa Observed band size : 146 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ABCB11 antibody - Carboxyterminal end (ab71793)
Formalin-fixed, paraffin-embedded human hepatocarcinoma tissue reacted with ab71793 at a 1:50 dilution, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
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Search PubMed (MEDLINE) for references to ABCB11
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ABCB11 antibody - Carboxyterminal end - more information
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