ABCB11 antibody - Carboxyterminal end (ab71793)

Product Name 

ABCB11 antibody - Carboxyterminal end

Product type 

Primary antibodies

Description 

Rabbit polyclonal to ABCB11 - Carboxyterminal end

Immunogen 

A KLH conjugated synthetic peptide selected from the C-terminal region of human ABCB11.

Reacts with 
(species key)

Hu, Ms

Tested applications 
(see key)

ELISA, IHC-P, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
ELISA: 1/1000.
IHC-P: 1/50 - 1/100.
WB: 1/100 - 1/500. Detects a band of approximately 146 kDa plus a range of other bands. (predicted molecular weight: 146 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Human hepatocarcinoma tissue and mouse liver tissue lysate.

Cellular localization 

Multi pass membrane protein

Research areas 

Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of lipids and lipoproteins
Signal Transduction >> Metabolism >> Lipid metabolism
Signal Transduction >> Metabolism >> Plasma Membrane >> Channels

Relevance 

Bile formation is an important function of the liver. It is mediated by hepatocytes which generate bile flow within the bile canaliculi by continuous vectorial secretion of bile salts and other solutes across their canalicular (apical) membrane. Bile secretion is mediated by several ATP-binding cassette (ABC) transporters located in the canalicular membrane of hepatocytes. Among these ABC transporters, ABCB11 (bile salt export pump or BSEP) represents the primary, if not sole transport system for the canalicular excretion of bile salts. Bile secretory failure results in cholestasis and progressive familial intrahepatic cholestasis (PFIC) in infancy represents a group of inherited cholestatic diseases that are classified into three subtypes. One of these subtypes, PFIC II, is associated with mutations in the ABCB11 gene. PFIC patients with mutations in the ABCB11 gene have normal serum gamma-glutamyltransferase activity, low concentrations of bile salts in bile, and an absence of bile duct proliferation. Additionally, human obesity is associated with altered cholesterol homeostasis including increased production and turnover, as well as secretion of excess cholesterol from the liver into bile.
ABCB11 is a multifunctional polypeptide with two homologous halves, each containing a hydrophobic membrane ancoring domain and an ATP binding cassette (ABC) domain. The membrane anchoring domain is composed of six helixes buried in the lipid bilayer of the plasma membrane and the ATP binding ABCs are exposed to the cytosol. The membrane anchoring domain helixes are thought to form channels spanning the plasma membrane.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Protein G purified

Storage buffer 

Preservative: 0.09% Sodium Azide
Constituents: PBS

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Purification notes 

This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Form 

Liquid

Concentration 

0.250 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

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