Anti-ABCB11/BSEP antibody (ab155421)
Key features and details
- Rabbit polyclonal to ABCB11/BSEP
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-ABCB11/BSEP antibody
See all ABCB11/BSEP primary antibodies -
Description
Rabbit polyclonal to ABCB11/BSEP -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human ABCB11/BSEP aa 474-745 (internal sequence).
Database link: O95342 -
Positive control
- Jurkat whole cell lysate (ab7899), AGS xenograft, A431 cells.
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General notes
Keep as concentrated solution.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab155421 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 146 kDa.
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IHC-P | (1) |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 146 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/1000. |
Target
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Function
Involved in the ATP-dependent secretion of bile salts into the canaliculus of hepatocytes. -
Tissue specificity
Expressed predominantly, if not exclusively in the liver, where it was further localized to the canalicular microvilli and to subcanalicular vesicles of the hepatocytes by in situ. -
Involvement in disease
Defects in ABCB11 are the cause of progressive familial intrahepatic cholestasis type 2 (PFIC2) [MIM:601847]. PFIC2 is an inherited liver disease of childhood which is characterized by cholestasis and normal serum gamma-glutamyltransferase activity. Defects in ABCB11 are also found in cases of chronic intrahepatic cholestasis without obvious familial history of chronic liver disease.
Defects in ABCB11 are the cause of benign recurrent intrahepatic cholestasis type 2 (BRIC2) [MIM:605479]. BRIC is characterized by intermittent episodes of cholestasis without progression to liver failure. There is initial elevation of serum bile acids, followed by cholestatic jaundice which generally spontaneously resolves after periods of weeks to months. The cholestatic attacks vary in severity and duration and patients are asymptomatic between episodes, both clinically and biochemically. -
Sequence similarities
Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily.
Contains 2 ABC transmembrane type-1 domains.
Contains 2 ABC transporter domains. -
Domain
Multifunctional polypeptide with two homologous halves, each containing an hydrophobic membrane-anchoring domain and an ATP binding cassette (ABC) domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 8647 Human
- Omim: 603201 Human
- SwissProt: O95342 Human
- Unigene: 658439 Human
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Alternative names
- ABC member 16, MDR/TAP subfamily antibody
- ABC16 antibody
- ABCB1 antibody
see all
Images
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Immunohistochemical analysis of paraffin embedded AGS xenograft labeling ABCB11/BSEP with ab155421 antibody at 1/100.
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Anti-ABCB11/BSEP antibody (ab155421) at 1/1000 dilution + Jurkat whole cell
lysate at 30 µg
Predicted band size: 146 kDa
5% SDS PAGE -
Immunofluorescent analysis of methanol-fixed A431 cells labeling ABCB11/BSEP with ab155421 at 1/500. Image at the right costained with Hoechst 33342.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (10)
ab155421 has been referenced in 10 publications.
- Liu M et al. Peptide modified geniposidic acid targets bone and effectively promotes osteogenesis. J Orthop Translat 38:23-31 (2023). PubMed: 36313979
- Wang W et al. Transcriptome and Gut Microbiota Profiling Analysis of ANIT-Induced Cholestasis and the Effects of Da-Huang-Xiao-Shi Decoction Intervention. Microbiol Spectr 10:e0324222 (2022). PubMed: 36409145
- Gao YD et al. [Cytotoxicity and underlying mechanism of evodiamine in HepG2 cells]. Beijing Da Xue Xue Bao Yi Xue Ban 53:1107-1114 (2021). PubMed: 34916690
- Liu L et al. Naturally Occurring TPE-CA Maintains Gut Microbiota and Bile Acids Homeostasis via FXR Signaling Modulation of the Liver-Gut Axis. Front Pharmacol 11:12 (2020). PubMed: 32116693
- Wei X et al. Ethyl acetate extract of herpetospermum pedunculosum alleviates a-naphthylisothiocyanate-induced cholestasis by activating the farnesoid x receptor and suppressing oxidative stress and inflammation in rats. Phytomedicine 76:153257 (2020). PubMed: 32534360
- Yan Y et al. Phytochemicals protect L02 cells against hepatotoxicity induced by emodin via the Nrf2 signaling pathway. Toxicol Res (Camb) 8:1028-1034 (2019). PubMed: 32153769
- Naik PP et al. Autophagy regulates cisplatin-induced stemness and chemoresistance via the upregulation of CD44, ABCB1 and ADAM17 in oral squamous cell carcinoma. Cell Prolif 51:N/A (2018). PubMed: 29171106
- Gan L et al. Functional analysis of the correlation between ABCB11 gene mutation and primary intrahepatic stone. Mol Med Rep N/A:N/A (2018). PubMed: 30431138
- Mather Q et al. A novel protein expression signature differentiates benign lipomas from well-differentiated liposarcomas. Mol Clin Oncol 7:315-321 (2017). PubMed: 28781809
- Zhou JJ et al. Knockdown of long non-coding RNA HOTAIR sensitizes hepatocellular carcinoma cell to cisplatin by suppressing the STAT3/ABCB1 signaling pathway. Oncol Lett 14:7986-7992 (2017). PubMed: 29250186