Anti-ABCB4 antibody [P3II-26] (ab24108)

Dear Sir/Madam,

I was recently reading an article which uses one of your antibodies. I cannot seem to locate this product on your website and was wondering if you could point me in the right direction. The article states using:

"a mouse-monoclonal antibody against mammalian Pgp (C219; Abcam, Cambridge, MA, USA).... This antibody targets two Pgp epitopes that are largely conserved in the SMDR2 sequence"

Another issue you may be able to help me with is whether or not you have a primary antibody that would recognise a variety of pgp/mdr proteins (has to include MDR2 (human gene family ABCB1) and MRP1 (human gene family ABCC1)) i.e. it may bind a highly conserved region across the pgps.

I would really appreciate any help with the matter as I have been trawling through many of the antibodies available and comparing immunogens.

I hope you can help, thank you for your time

Kind regards

ANSWER:

Thank you for contacting us.

ab3364; anti P Glycoprotein is no longer available in our catalogue. However we have similar products available to buy. The catalogue numbers are

ab3083; ab3366; ab10333; ab80594; ab80626. Please check the datasheets of these antibodies and select a product according to your requirements.

The anti MDR2 products are ab71792; ab24108 and ab111209

Anti MRP1 products are ab24102; ab63987; ab99531; ab81903

To search different products please type target name in the search box provided on the Abcam homepage at www.abcam.com. After hitting the search button the system will give you the sorted product as per your target request.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

I will need to detect MDR3 protein in a stably transfected cell line by performing western blotting. I am considering to use P3II-26 (product No. ab24108) as the primary antibody. I wonder if you could help me with a couple of questions: 1. Did you test this antibody for use in a western blot application? 2. If yes, what did you use as the positive control and what's the dilution for this antibody?

ANSWER:

Thank you for your enquiry.

This antibody was tested in Western blot. I could not find a specific dilution recommended for this antibody, but in general we recommend using 1-20ug/ml of primary antibody, and this antibody is supplied as approximately 250 µg immunoglobulin/ml. Therefore, I would recommend starting at 1:100 and optimizing from there.

I would recommend liver tissue as a positive control, since the datasheet states that this protein mediates the translocation of phosphatidylcholine across the canalicular membrane of the hepatocyte.

I hope this information helps, please do not hesitate to contact us if you need any more advice or information.