ABCB4 antibody [P3II-26] (ab24108)

Product Name 

ABCB4 antibody [P3II-26]

Product type 

Primary antibodies

Description 

Mouse monoclonal [P3II-26] to ABCB4

Immunogen 

Fusion protein: NMQTSGSQIQ SEEFELNDEK AATRMAPNGW KSRLFRHSTQ KNLKNSQMCQ KSLDVETDGL EANV, corresponding to Internal sequence amino acids 629-692 of Human ABCB4.
BLAST 'NMQTSGSQIQ SEEFELNDE K AATRMAPN GW KSRLFRH STQ KNLKNS QMCQ KSLDV ETDGL EANV ' with or

Reacts with 
(species key)

Hu

Specificity 

Clone P3II-26 does not cross-react with the human ABCB1.

Epitope 

Clone P3II-26 reacts with an internal epitope of ABCB4.

Tested applications 
(see key)

Flow Cyt, ICC, IHC-Fr, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
Flow Cyt: Use at an assay dependent dilution.
ICC: 1/20 - 1/50 on acetone fixed cytospin preparations.
IHC-Fr: 1/20. Use on acetone-fixed frozen sections; can be followed by incubation with rabbit anti-mouse IgG and a monoclonal mouse APAAP complex.
WB: Use at an assay dependent dilution. Predicted molecular weight: 141 kDa.


Is unsuitable for IHC-P.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Integral membrane protein.

Research areas 

Signal Transduction >> Metabolism >> Lipid metabolism
Signal Transduction >> Metabolism >> Plasma Membrane >> ATPases
Signal Transduction >> Protein Trafficking >> Nuclear Import / Export

Relevance 

ABCB4 is a membrane-associated protein and member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. The gene encoding this protein is a member of the MDR/TAP subfamily of genes. Members of the MDR/TAP subfamily are involved in multidrug resistance as well as antigen presentation. ABCB4 is a member of the p-glycoprotein family of membrane proteins with phosphatidylcholine as its substrate.
ABCB4 is the energy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells, though it is not capable of conferring drug resistance. This protein mediates the translocation of phosphatidylcholine across the canalicular membrane of the hepatocyte.
Defects in ABCB4 are the cause of progressive familial intrahepatic cholestasis type III (PFIC3). PFIC3 is an autosomal recessive liver disorder presenting with early onset cholestasis that progresses to cirrhosis and liver failure before adulthood. It is characterized by elevated serum gamma-glutamyltransferase levels.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

P3II-26

Isotype 

IgG2b

Purity 

Tissue culture supernatant

Storage buffer 

Preservative: 0.1% Sodium Azide
Constituents: 0.7% BSA, serum-free tissue culture supernatant

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

0.250 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

References for ABCB4 antibody [P3II-26] (ab24108)

  This product has been used in:

Scheffer GL et al. Specific detection of multidrug resistance proteins MRP1, MRP2, MRP3, MRP5, and MDR3 P-glycoprotein with a panel of monoclonal antibodies. Cancer Res 60:5269-77 (2000). PubMed: 11016657
If you publish research using ab24108 please let us know so that we can cite the reference on this datasheet.

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