Anti-ABCB4 antibody [P3II-26] (ab24108)

Overview

• Product nameAnti-ABCB4 antibody [P3II-26]See all ABCB4 primary antibodies ...
• Description
  Mouse monoclonal [P3II-26] to ABCB4
• SpecificityClone P3II-26 does not cross-react with the human ABCB1.
• Tested applicationsICC, IHC-Fr, WB, Flow Cyt more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Fusion protein: NMQTSGSQIQ SEEFELNDEK AATRMAPNGW KSRLFRHSTQ KNLKNSQMCQ KSLDVETDGL EANV, corresponding to Internal sequence amino acids 629-692 of Human ABCB4.

  Run BLAST with Run BLAST with
• EpitopeClone P3II-26 reacts with an internal epitope of ABCB4.

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.1% Sodium Azide
  Constituents: 0.7% BSA, serum-free tissue culture supernatant
• PurityTissue culture supernatant
• Clonality Monoclonal
• Clone numberP3II-26
• IsotypeIgG2b
• Research Areas
  • Metabolism
  • Types of disease
  • Cancer

  • Signal Transduction
  • Metabolism
  • Plasma Membrane
  • ATPases

  • Signal Transduction
  • Metabolism
  • Lipid metabolism

  • Signal Transduction
  • Protein Trafficking
  • Nuclear Import / Export

Applications

Target

• FunctionMediates ATP-dependent export of organic anions and drugs from the cytoplasm. Hydrolyzes ATP with low efficiency. Human MDR3 is not capable of conferring drug resistance. Mediates the translocation of phosphatidylcholine across the canalicular membrane of the hepatocyte.
• Involvement in diseaseDefects in ABCB4 are the cause of progressive familial intrahepatic cholestasis type 3 (PFIC3) [MIM:602347]. PFIC3 is an autosomal recessive liver disorder presenting with early onset cholestasis that progresses to cirrhosis and liver failure before adulthood. It is characterized by elevated serum gamma-glutamyltransferase levels.
  Defects in ABCB4 are a cause of intrahepatic cholestasis of pregnancy (ICP) [MIM:147480]; also known as obstetric cholestasis. ICP is a multifactorial liver disorder of pregnancy. It presents during the second or, more commonly, the third trimestre of pregnancy with intense pruritus which becomes more severe with advancing gestation and cholestasis. Cholestasis results from abnormal biliary transport from the liver into the small intestine. ICP causes fetal distress, spontaneous premature delivery and intrauterine death. ICP patients have spontaneous and progressive disappearance of cholestasis after delivery.
  Defects in ABCB4 are a cause of gallbladder disease type 1 (GBD1) [MIM:600803]. It is one of the major digestive diseases. Gallstones composed of cholesterol (cholelithiasis) are the common manifestations in western countries. Most people with gallstones, however, remain asymptomatic through their lifetimes.
• Sequence similaritiesBelongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily.
  Contains 2 ABC transmembrane type-1 domains.
  Contains 2 ABC transporter domains.
• Cellular localizationCell membrane.

Target information above from: UniProt accession P21439 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 5244 Human
  • Omim: 171060 Human
  • SwissProt: P21439 Human
  • Unigene: 654403 Human

• Alternative names
  ABC 21 antibodyABC B4 antibodyABC21 antibody

  ABCB 4 antibodyAbcb4 antibodyABCB4 protein antibodyATP binding cassette sub family B MDR/TAP member 4 antibodyATP binding cassette sub family B member 4 antibodyATP-binding cassette sub-family B member 4 antibodyMDR 3 antibodyMDR2 antibodyMDR2/3 antibodyMDR3 antibodyMDR3 P glycoprotein antibodyMDR3 P gp antibodyMDR3_HUMAN antibodyMultidrug resistance protein 3 antibodyMultiple drug resistance 3 antibodyP glycoprotein 3 antibodyP-glycoprotein 3 antibodyPFIC 3 antibodyPFIC3 antibodyPGY 3 antibodyPGY3 antibody

  see all

Anti-ABCB4 antibody [P3II-26] images

• 

  Flow Cytometry-Anti-ABCB4 antibody [P3II-26](ab24108)
  Overlay histogram showing HeLa cells stained with ab24108 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab24108, 1µg/1x10⁶ cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.