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Anti-ABCB4 antibody (ab71792) at 1/100 dilution + Mouse heart tissue lysates at 12.5 µg
Secondary
HRP-anti-rabbit
Predicted band size : 141 kDa
Observed band size : 170 kDa (why is the actual band size different from the predicted?)
Additional bands at : 40& 43 kDa,47 kDa,75 kDa. We are unsure as to the identity of these extra bands.
Formalin-fixed, paraffin-embedded human hepatocarcinoma tissue reacted with ab71792 at a 1:50 dilution, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
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